Research question

Ovarian stimulation during IVF cycles involves close monitoring of oestradiol, progesterone and ultrasound measurements of follicle growth. In contrast to blood draws, sampling saliva is less invasive. Here, a blind validation is presented of a novel saliva-based oestradiol and progesterone assay carried out in samples collected in independent IVF clinics.

Design

Concurrent serum and saliva samples were collected from 324 patients at six large independent IVF laboratories. Saliva samples were frozen and run blinded. A further 18 patients had samples collected more frequently around the time of HCG trigger. Saliva samples were analysed using an immunoassay developed with Salimetrics LLC.

Results

In total, 652 pairs of saliva and serum oestradiol were evaluated, with correlation coefficients ranging from 0.68 to 0.91. In the European clinics, a further 237 of saliva and serum progesterone samples were evaluated; however, the correlations were generally poorer, ranging from –0.02 to 0.22. In the patients collected more frequently, five out of 18 patients (27.8%) showed an immediate decrease in oestradiol after trigger. When progesterone samples were assessed after trigger, eight out of 18 (44.4%) showed a continued rise.

Conclusions

Salivary oestradiol hormone testing correlates well to serum-based assessment, whereas progesterone values, around the time of trigger, are not consistent from patient to patient.

中文翻译：

---

ART 监测中唾液激素雌二醇和孕酮测量的多中心国际研究

研究问题

IVF 周期期间的卵巢刺激包括密切监测雌二醇、孕酮和卵泡生长的超声测量值。与抽血相比，唾液采样的侵入性较小。在这里，对在独立试管婴儿诊所收集的样本中进行的新型唾液雌二醇和孕酮检测进行了盲验证。

设计

同时收集了来自六个大型独立试管婴儿实验室的 324 名患者的血清和唾液样本。唾液样品被冷冻并在盲法下运行。另有 18 名患者在 HCG 触发时间前后更频繁地收集样本。使用由 Salimetrics LLC 开发的免疫测定法分析唾液样品。

结果

总共评估了 652 对唾液和血清雌二醇，相关系数从 0.68 到 0.91。在欧洲诊所，对另外 237 份唾液和血清孕酮样本进行了评估；然而，相关性通常较差，范围从 –0.02 到 0.22。在更频繁收集的患者中，18 名患者中有 5 名 (27.8%) 在触发后雌二醇立即下降。当在触发后评估黄体酮样本时，18 个中有 8 个（44.4%）显示持续上升。

结论

唾液雌二醇测试与基于血清的评估密切相关，而孕酮值在触发时间前后因患者而异。