Introduction

Responses of oral-microflora-exposed dental pulp to a triple antibiotic paste (TAP), a mixture of ciprofloxacin, metronidazole, and minocycline in ointment with macrogol and propylene glycol, remain to be fully clarified at the cellular level. This study aimed to elucidate responses of oral-microflora-exposed dental pulp to capping with TAP in mouse molars.

Methods

A cavity was prepared on the first molars of 6-week-old mice to expose the dental pulp for 24 h. The exposed pulp was capped with TAP (TAP group) or calcium hydroxide cement (CH group), in addition to the combination of macrogol (M) and propylene glycol (P) (MP, control group), followed by a glass ionomer cement filling. The samples were collected at intervals of 1, 2, and 3 weeks, and immunohistochemistry for nestin and Ki-67 and deoxyuride-5′-triphosphate biotin nick end labeling (TUNEL) assay were performed in addition to quantitative real-time polymerase chain reaction (qRT-PCR) analyses.

Results

The highest occurrence rate of pulp necrosis was found in the control group followed by the CH group at Weeks 2 and 3, whereas the highest occurrence rate of healed areas in the dental pulp was observed in the TAP group at each time point. Tertiary dentin formation was first observed in the dental pulp of the TAP group at Week 2. In contrast, bone-like and/or fibrous tissues were frequently observed in the CH group. qRT-PCR analyses clarified that TAP activated the stem and dendritic cells at Weeks 1 and 2, respectively.

Conclusions

The use of TAP as a pulp-capping agent improved the healing process of oral-microflora-exposed dental pulp in mouse molars.

中文翻译：

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暴露于口腔微生物区系的牙髓对小鼠磨牙三联抗生素糊剂或氢氧化钙粘固剂封端的反应

介绍

暴露于口腔微生物区系的牙髓对三重抗生素糊剂 (TAP) 的反应，即环丙沙星、甲硝唑和米诺环素与聚乙二醇和丙二醇软膏的混合物，仍有待在细胞水平上完全阐明。本研究旨在阐明暴露于口腔微生物区系的牙髓对小鼠臼齿 TAP 封盖的反应。

方法

在 6 周龄小鼠的第一磨牙上制备空腔，暴露牙髓 24 小时。除了聚乙二醇 (M) 和丙二醇 (P) 的组合（MP，对照组）外，暴露的牙髓用 TAP（TAP 组）或氢氧化钙水泥（CH 组）封盖，然后填充玻璃离子水门汀. 以 1、2 和 3 周的间隔收集样本，除了定量实时聚合酶链反应外，还进行了巢蛋白和 Ki-67 的免疫组织化学和脱氧尿苷-5'-三磷酸生物素缺口末端标记 (TUNEL) 测定(qRT-PCR) 分析。

结果

在第 2 周和第 3 周时，对照组牙髓坏死发生率最高，其次是 CH 组，而 TAP 组在每个时间点观察到牙髓愈合区域发生率最高。在第 2 周，TAP 组的牙髓中首次观察到三次牙本质形成。相比之下，在 CH 组中经常观察到骨样和/或纤维组织。qRT-PCR 分析阐明 TAP 分别在第 1 周和第 2 周激活了干细胞和树突细胞。

结论

使用 TAP 作为盖髓剂改善了小鼠臼齿中暴露于口腔微生物区系的牙髓的愈合过程。