Synthetic polymer hydrogel nanoparticles (NPs) were developed to function as abiotic affinity reagents for fibrinogen. These NPs were made using both temperature-sensitive N-isopropyl acrylamide (NIPAm) and l-amino acid monomers. Five kinds of l-amino acids were acryloylated to obtain functional monomers: l-phenylalanine (Phe) and l-leucine (Leu) with hydrophobic side chains, l-glutamic acid (Glu) with negative charges, and l-lysine (Lys) and l-arginine (Arg) with positive charges. After incubating the NPs with fibrinogen, γ-globulin, and human serum albumin (HSA) respectively, the NPs that incorporated N-acryloyl-Arg monomers (AArg@NPs) showed the strongest and most specific binding affinity to fibrinogen, when compared with γ-globulin and HSA. Additionally, the fibrinogen-AArg binding model had the best docking scores, and this may be due to the interaction of positively charged AArg@NPs and the negatively charged fibrinogen D domain and the hydrophobic interaction between them. The specific adsorption of AArg@NPs to fibrinogen was also confirmed by the immunoprecipitation assay, as the AArg@NPs selectively trapped the fibrinogen from a human plasma protein mixture. AArg@NPs had a strong selectivity for, and specificity to, fibrinogen and may be developed as a potential human fibrinogen-specific affinity reagent.

合成聚合物水凝胶纳米粒子 (NPs) 被开发用作纤维蛋白原的非生物亲和试剂。这些纳米颗粒是使用温度敏感的 N-异丙基丙烯酰胺 (NIPAm) 和l-氨基酸单体制成的。五种l-氨基酸被丙烯酰化得到功能单体：l-苯丙氨酸（Phe）和l-亮氨酸（Leu）具有疏水侧链，l-谷氨酸（Glu）带负电荷，l-赖氨酸（Lys）和l-精氨酸 (Arg) 带正电荷。将 NPs 分别与纤维蛋白原、γ-球蛋白和人血清白蛋白 (HSA) 孵育后，与 γ 相比，掺入 N-丙烯酰-精氨酸单体 (AArg@NPs) 的 NPs 对纤维蛋白原表现出最强和最特异性的结合亲和力-球蛋白和 HSA。此外，纤维蛋白原-AArg 结合模型的对接得分最高，这可能是由于带正电的 AArg@NPs 与带负电的纤维蛋白原 D 结构域的相互作用以及它们之间的疏水相互作用。AArg@NPs 对纤维蛋白原的特异性吸附也通过免疫沉淀试验得到证实，因为 AArg@NPs 选择性地从人血浆蛋白混合物中捕获纤维蛋白原。AArg@NPs 具有很强的选择性和特异性，