DNA-nanotechnology-based nano-architecture scaffolds based on circular strands were designed in the form of DNA-nanowires (DNA-NWs) as a polymer of DNA-triangles. Circularizing a scaffold strand (84-NT) was the critical step followed by annealing with various staple strands to make stiff DNA-triangles. Atomic force microcopy (AFM), native polyacrylamide gel electrophoresis (PAGE), UV-analysis, MTT-assay, flow cytometry, and confocal imaging were performed to assess the formulated DNA-NWs and cisplatin (CPT) loading. The AFM and confocal microscopy images revealed a uniform shape and size distribution of the DNA-NWs, with lengths ranging from 2 to 4 μm and diameters ranging from 150 to 300 nm. One sharp band at the top of the lane (500 bp level) with the loss of electrophoretic mobility during the PAGE (native) gel analysis revealed the successful fabrication of DNA-NWs. The loading efficiency of CPT ranged from 66.85% to 97.35%. MTT and flow cytometry results showed biocompatibility of the blank DNA-NWs even at 95% concentration compared with the CPT-loaded DNA-NWs. The CPT-loaded DNA-NWs exhibited enhanced apoptosis (22%) compared to the apoptosis (7%) induced by the blank DNA-NWs. The release of CPT from the DNA-NWs was sustained at < 75% for 6 h in the presence of serum, demonstrating suitability for systemic applications. The IC₅₀ of CPT@DNA-NWs was reduced to 12.8 nM CPT, as compared with the free CPT solution exhibiting an IC₅₀ of 51.2 nM. Confocal imaging revealed the targetability, surface binding, and slow internalization of the DNA-NWs in the scavenger-receptor-rich cancer cell line (HepG2) compared with the control cell line.

基于 DNA 纳米技术的纳米结构支架以圆形链为基础，设计为 DNA 纳米线 (DNA-NW) 的形式，作为 DNA 三角形的聚合物。环化支架链 (84-NT) 是关键步骤，然后用各种主链退火以形成坚硬的 DNA 三角形。进行原子力显微镜 (AFM)、天然聚丙烯酰胺凝胶电泳 (PAGE)、UV 分析、MTT 测定、流式细胞术和共焦成像来评估配制的 DNA-NW 和顺铂 (CPT) 负载。 AFM 和共焦显微镜图像显示 DNA-NW 具有均匀的形状和尺寸分布，长度范围为 2 至 4 μm，直径范围为 150 至 300 nm。在 PAGE（天然）凝胶分析过程中，泳道顶部出现一条尖锐的条带（500 bp 水平），并且电泳迁移率丧失，这表明 DNA-NW 的成功制造。 CPT的加载效率范围为66.85%至97.35%。 MTT 和流式细胞术结果显示，与负载 CPT 的 DNA-NW 相比，即使在 95% 浓度下，空白 DNA-NW 也具有生物相容性。与空白 DNA-NW 诱导的细胞凋亡 (7%) 相比，负载 CPT 的 DNA-NW 表现出增强的细胞凋亡 (22%)。在血清存在的情况下，DNA-NW 中 CPT 的释放在 < 75% 下持续 6 小时，证明了其适合全身应用。与 IC ₅₀为 51.2 nM 的游离 CPT 溶液相比，CPT@DNA-NWs 的 IC ₅₀降低至 12.8 nM CPT。共聚焦成像揭示了与对照细胞系相比，富含清道夫受体的癌细胞系 (HepG2) 中 DNA-NW 的靶向性、表面结合和缓慢内化。