Abstract In this work, 4-pentenoyl-alanyl-chitosan oligosaccharide (PACO), a functional oligomer of chitosan oligosaccharide with the strong affinity on curcumin (CUR), was screened from the series of 4-pentenoyl-aminoacyl-chitosan oligosaccharides through the results of computational selection. After the preparation of this oligomer, a novel molecular imprinting membrane-based electrochemical sensor was developed for sensitive detection of CUR. The molecularly imprinted membrane (MIM) of CUR was obtained by composite compensation polymerization process: bulk polymerization with good permeability on a glassy carbon electrode (GCE) surface by using CUR as the template molecule, PACO as the oligomer of functional monomer and ethylene glycol dimethacrylate as the crosslinking agent, followed by electrochemical polymerization with good sensitivity in methylene bisacrylamide solution. After removing the template molecule, the modified electrode could specifically adsorb CUR molecules. Under optimal conditions, the sensor named as CUR-MIM/GCE exhibited high sensitivity and good selectivity against some analogs of CUR such as tetrahydrocurcumin, ferulic acid, β-carotene, and quercetin. The calibration graph of the designed sensor was linear in the range of 10 nM to 2.0 μM with a detection limit of 5.0 nM. This sensor showed excellent selectivity and good reproducibility, and more than 90% of its original response was retained after storage in a sealed capsule at room temperature for 30 days. Moreover, the constructed sensor was successfully applied to monitor the CUR contents in turmeric extract with the relative standard deviation of less than 3.2%.

中文翻译：

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以4-戊烯酰基-氨基酰基-壳聚糖寡糖为功能单体低聚物的复合聚合分子印迹膜基电化学传感器灵敏测定姜黄素

摘要 本研究从4-戊烯酰-氨酰-壳聚糖寡糖系列中筛选出4-戊烯酰-丙氨酰-壳聚糖寡糖(PACO)，一种对姜黄素(CUR)具有强亲和力的功能性壳寡糖低聚物。的计算选择。在制备这种低聚物后，开发了一种新型的基于分子印迹膜的电化学传感器，用于灵敏检测 CUR。通过复合补偿聚合工艺获得CUR的分子印迹膜（MIM）：以CUR为模板分子，PACO为功能单体与乙二醇二甲基丙烯酸酯的低聚物，在玻碳电极（GCE）表面进行具有良好渗透性的本体聚合作为交联剂，然后在亚甲基双丙烯酰胺溶液中以良好的灵敏度进行电化学聚合。去除模板分子后，修饰电极可以特异性吸附CUR分子。在最佳条件下，被命名为 CUR-MIM/GCE 的传感器对 CUR 的一些类似物如四氢姜黄素、阿魏酸、β-胡萝卜素和槲皮素表现出高灵敏度和良好的选择性。所设计传感器的校准曲线在 10 nM 至 2.0 μM 范围内呈线性，检测限为 5.0 nM。该传感器显示出优异的选择性和良好的重现性，在室温下密封胶囊中储存 30 天后，其原始响应的 90% 以上保留。而且，