Abstract A study of biosensors formed with the enzyme horseradish peroxidase immobilized onto gold electrodes with cyclic voltammetry is presented here. This enzyme was chemically linked to self-assembled monolayers of 3-mercaptopropionic, 8-mercaptooctanoic and 11-mercaptoundecanoic acids. Gold electrodes were used as holders. The goal of this work was the determination of the optimum concentration of the enzyme contained in the deposition solution that was used to modify the carboxylic ending of the monolayers just to form the biosensors. This concentration was established at 5, 7 and 10 μmol L−1 for the 11-mercaptoundecanoic, the 8-mercaptooctanoic and the 3-mercaptopropanoic acids respectively. UV–visible spectroscopy experiments allowed this fact. Furthermore, the good quality of the formation of the thiols monolayers was confirmed by using potassium ferricyanide as an electrochemical probe and reductive desorption voltammograms. Additionally, the substrate guaiacol was employed successfully to validate the developed biosensors. To conclude, comparisons between the three monolayers suggested that the shortest monolayer was the less compact, although the one with the best sensibility.

中文翻译：

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固定在自组装单层上的辣根过氧化物酶生物传感器：沉积酶浓度的优化

摘要 这里介绍了用循环伏安法对固定在金电极上的辣根过氧化物酶形成的生物传感器的研究。这种酶与 3-巯基丙酸、8-巯基辛酸和 11-巯基十一酸的自组装单层化学连接。金电极用作支架。这项工作的目标是确定沉积溶液中所含酶的最佳浓度，该酶用于修饰单层的羧基末端以形成生物传感器。对于 11-巯基十一烷酸、8-巯基辛酸和 3-巯基丙酸，该浓度分别确定为 5、7 和 10 μmol L-1。紫外-可见光谱实验证实了这一事实。此外，通过使用铁氰化钾作为电化学探针和还原解吸伏安图证实了硫醇单层形成的良好质量。此外，底物愈创木酚已成功用于验证开发的生物传感器。总而言之，三个单层之间的比较表明，最短的单层是不太紧凑的，尽管具有最好的敏感性。