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Efficient extraction and preservation of thermotolerant phycocyanins from red alga Cyanidioschyzon merolae
Journal of Bioscience and Bioengineering ( IF 2.8 ) Pub Date : 2020-10-24 , DOI: 10.1016/j.jbiosc.2020.09.021
Chihiro Yoshida , Miyabi Murakami , Anna Niwa , Masahiro Takeya , Takashi Osanai

C-Phycocyanin (PC) is a protein used commercially as a natural blue pigment produced by cyanobacteria, cryptophytes, and rhodophytes. Although it is industrially synthesized from the cyanobacterium Arthrospira platensis, PC requires high levels of energy for its extraction, which involves freezing of cells. However, as a protein, PC is easily denatured at extreme temperatures. In this study, we extracted PC from the red alga Cyanidioschyzon merolae, denoted as CmPC, and found that this protein was tolerant to high temperatures and acidic pH. CmPC was extracted by suspending cells in water mixed with various salts and organic acids without freeze-drying or freeze-thaw. The stability of CmPC varied with salt concentration and was destabilized by organic acids. Our results indicate that C. merolae is a potential candidate for PC production with thermotolerant properties.



中文翻译:

从红藻Cyanidioschyzon merolae高效提取和保存耐热藻蓝蛋白

C-藻蓝蛋白(C-Phycocyanin(PC))是一种商业上用作由蓝藻,隐藻和红藻产生的天然蓝色颜料的蛋白质。尽管PC是从蓝细菌Arthrospira platensis工业合成的,但PC提取所需的能量很高,这涉及细胞的冻结。但是,PC作为蛋白质,在极端温度下容易变性。在这项研究中,我们从名为CmPC的红藻Cyanidioschyzon merolae中提取了PC ,发现该蛋白可以耐受高温和酸性pH。通过将细胞悬浮在与各种盐和有机酸混合的水中而无需冷冻干燥或冻融的方法来提取CmPC。CmPC的稳定性随盐浓度而变化,并被有机酸破坏。我们的结果表明C. merolae是具有耐热特性的PC生产的潜在候选者。

更新日期:2020-10-24
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