Previously, we reported that a dairy-originated probiotic bacterium, Propionibacterium freudenreichii subsp. freudenreichii B3523 (PF) reduced cecal colonization of multidrug-resistant Salmonella enterica serovar Heidelberg (SH) in poults (2-wk-old) and growing (7-wk-old) turkeys. In the current study, we validated the antibacterial potential of PF in finisher (12-wk-old) turkeys using two separate experiments. In each experiment, 1-d old poults were randomly allocated to three treatment groups, with 10 turkeys in each group (N=30 turkeys/experiment). The treatment groups were a negative control (NC; turkeys without PF supplementation or SH challenge), an SH control (SC; turkeys challenged with SH without PF supplementation) and a test group (PFS; turkeys supplemented with PF and challenged with SH). Supplementation of PF at 10¹² CFU/ 5 gallons water was provided to the turkeys in the PFS groups for 12 wk. The turkeys in the SC and PFS groups were challenged with SH at 10⁸ CFU/turkey at 11 wk of age. Five turkeys from each group were euthanized on 2- and 7- d after SH challenge. The cecum, liver, and spleen samples were collected to determine colonization of SH in the cecum and SH dissemination to the targeted internal organs. PF was recovered at 4.0 log₁₀ CFU/g in turkey cecum. Supplementation of PF reduced the cecal colonization of SH by >2.0 log₁₀ CFU/g in finishing turkeys (P<0.05). Similarly, PF supplementation reduced SH dissemination to the spleen (P<0.05). Our investigations revealed that PF could be a biocontrol strategy to reduce Salmonella colonization and its spread to internal organs in turkeys.

以前，我们报道了一种源自乳制品的益生菌，即丙酸丙酸杆菌亚种。freudenreichii B3523（PF）减少了对多药耐药小肠沙门氏菌血清型海德堡（SH）在家禽（2周龄）和生长中（7周龄）火鸡的盲肠定植。在当前的研究中，我们使用两个单独的实验验证了肥育期（12周龄）火鸡中PF的抗菌潜力。在每个实验中，将1天大的家禽随机分配到三个治疗组，每组10只火鸡（N = 30只火鸡/实验）。治疗组为阴性对照（NC；未添加PF或未进行SH攻击的火鸡），SH对照（SC; 火鸡在不添加PF的情况下接受SH攻击）和一个测试组（PFS；火鸡在PF情况下进行SH挑战）。PFS组的火鸡在12周内补充了10 ¹² CFU / 5加仑水的PF 。SC和PFS组的火鸡在11 wk时分别以10 ⁸ CFU /火鸡和SH攻击。在SH攻击后第2天和第7天将每组的五只火鸡安乐死。收集盲肠，肝和脾样品，以确定盲肠中SH的定植和SH向靶标内脏的扩散。在土耳其盲肠中以4.0 log ₁₀ CFU / g的浓度回收PF 。补充PF可使SH的盲肠定植量减少> 2.0 log ₁₀土耳其火鸡CFU / g（P <0.05）。同样，PF的添加减少了SH向脾的扩散（P <0.05）。我们的研究表明，PF可能是减少沙门氏菌定植及其在火鸡内部器官中传播的一种生物控制策略。