Introduction and purpose

Indoleamine 2, 3- dioxygenase (IDO) plays an important role in immunosuppressive pathway, as inhibits responses of T cells and promotes immune tolerance. Host response to Helicobacter pylori (H. pylori) is involved in the infection persistence and it is also associated with different clinical outcomes. The aim of this study was to investigate the role of IDO in H. pylori-infected patients with gastritis diseases and peptic ulcer diseases (PUD) through the assessment of the relationship among IDO protein expression and the numbers of T helper (Th)-1, Th17, Th22, and T regulator (Treg) cells.

Materials and methods

Antrum biopsy was obtained from H. pylori-negative patients (n = 48) and H. pylori-positive subjects (55 patients with gastritis and 47 patients with PUD), for performing H. pylori status and histopathological assessments. IDO protein expression was evaluated by Western blotting.

Results

IDO protein expression was significantly higher in gastric biopsies from H. pylori-positive subjects compared to the H. pylori-negative subjects, and also in H. pylori-positive subjects with gastritis disease compared to H. pylori-positive subjects with PUD. Moreover, in H. pylori-positive subjects, a positive correlation was observed between IDO protein expression and the frequency of Treg cells. In addition, a negative correlation was observed between IDO protein expression and the number of Th1, Th17, and Th22.

Conclusion

Increased IDO protein expression is able to change the number of Th1, Th17, Th22, and Treg cells and these changes are possibly associated with an increase in the risk of PUD development in H. pylori-infected patients.

中文翻译：

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幽门螺杆菌感染胃粘膜中吲哚胺 2, 3-双加氧酶表达增加可调节 Th1/Th17/Th22 和 Treg 通路

 简介和目的

吲哚胺2,3-双加氧酶（IDO）在免疫抑制途径中发挥着重要作用，可抑制T细胞的反应并促进免疫耐受。宿主对幽门螺杆菌（ H. pylori ）的反应与感染持续性有关，并且还与不同的临床结果相关。本研究的目的是通过评估 IDO 蛋白表达与辅助性 T (Th)-1 数量之间的关系，探讨 IDO 在幽门螺杆菌感染的胃炎疾病和消化性溃疡疾病 (PUD) 患者中的作用。 、Th17、Th22 和 T 调节细胞 (Treg)。

 材料和方法

从幽门螺杆菌阴性患者 (n = 48) 和幽门螺杆菌阳性受试者 (55 名胃炎患者和 47 名 PUD 患者) 获取胃窦活检，用于进行幽门螺杆菌状态和组织病理学评估。通过蛋白质印迹评估IDO蛋白表达。

 结果

与幽门螺杆菌阴性受试者相比，幽门螺杆菌阳性受试者的胃活检中 IDO 蛋白表达显着较高，并且患有胃炎疾病的幽门螺杆菌阳性受试者中的 IDO 蛋白表达也显着高于患有 PUD 的幽门螺杆菌阳性受试者。此外，在幽门螺杆菌阳性受试者中，观察到IDO蛋白表达与Treg细胞频率之间呈正相关。此外，IDO蛋白表达与Th1、Th17和Th22的数量呈负相关。

 结论

IDO蛋白表达增加能够改变Th1、Th17、Th22和Treg细胞的数量，并且这些变化可能与幽门螺杆菌感染患者中PUD发展风险的增加有关。