Fungal growth often appears in a surrounding where water and nutrients are scarce. The impact of this environment during sporogenesis on subsequent growth is often neglected. This study investigates the effect of water availability during sporogenesis on subsequent early growth. Therefore, a carbon-depleted substrate was constructed. Humidity is then the only parameter of interest. The water conditions during sporogenesis, and during subsequent growth, were varied. This is a stressing environment: no carbon source is present, and water provided solely via the vapour. The lag time, tl, and initial growth rate, μfp, of the germ tubes were monitored. The effect of aw history on germination and initial growth depends on the RH of the environment. Only at low RH do spores produced at low aw have a smaller tl and higher μfp compared to those grown at high aw. This result was remarkably pronounced when the substrate was also made hydrophobic: growth only occurred when spores were developed at low aw and placed in high RH. Spores grown on lowered aw attract more water. It is hypothesized that this attraction affects subsequent growth behaviour, and is the reason why growth on hydrophobic glass only prevails in the condition of high RH and lowered aw history. We demonstrate the influence of cultivation conditions on germination, which becomes more pronounced in a more desiccated environment.

中文翻译：

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Penicillium rubens 在干燥和营养耗尽的条件下发芽取决于孢子发生过程中的水分活度

真菌生长通常出现在水和养分稀缺的环境中。这种环境在孢子发生过程中对后续生长的影响常常被忽视。这项研究调查了孢子发生过程中水的可用性对随后的早期生长的影响。因此，构建了贫碳基板。湿度是唯一感兴趣的参数。孢子发生期间和随后的生长期间的水条件各不相同。这是一个压力很大的环境：不存在碳源，水仅通过蒸汽提供。监测胚芽管的滞后时间 tl 和初始生长速率 μfp。aw 历史对发芽和初始生长的影响取决于环境的相对湿度。与在高 aw 下生长的孢子相比，仅在低 RH 下，低 aw 下产生的孢子具有更小的 tl 和更高的 μfp。当底物也具有疏水性时，这一结果非常明显：仅当孢子在低 aw 下发育并置于高 RH 中时才会发生生长。在较低的 aw 上生长的孢子会吸引更多的水。据推测，这种吸引力会影响随后的生长行为，这也是为什么在疏水性玻璃上的生长仅在高 RH 和低 aw 历史条件下盛行的原因。我们展示了栽培条件对发芽的影响，这在更干燥的环境中变得更加明显。在较低的 aw 上生长的孢子会吸引更多的水。据推测，这种吸引力会影响随后的生长行为，这也是为什么在疏水性玻璃上的生长仅在高 RH 和低 aw 历史条件下盛行的原因。我们展示了栽培条件对发芽的影响，这在更干燥的环境中变得更加明显。在较低的 aw 上生长的孢子会吸引更多的水。据推测，这种吸引力会影响随后的生长行为，这也是为什么在疏水性玻璃上的生长仅在高 RH 和低 aw 历史条件下盛行的原因。我们展示了栽培条件对发芽的影响，这在更干燥的环境中变得更加明显。