Yersinia enterocolitica bio-serotype 4/O:3 was previously identified in a pork production chain in Brazil and the obtained isolates presented high identity by pulsed-field gel electrophoresis (PFGE, XbaI). For the current study, an additional 147 porcine samples (tonsils = 100, palate = 30, head meat = 17) were collected from the same pork production chain 2-years later and 14 (9.5%) tested positive for Y. enterocolitica. Isolates (n = 24, 1 to 2 per positive sample) were bio-serotype 4/O:3 and harbored virulence genes ail, inv, wbbU, virF, myfA, ystA, ymoA, hreP and sat, and the multidrug resistance related genes emrD, marC and yfhD. PFGE (XbaI) demonstrated no differences among isolates (100% similarity) and were identical to some Y. enterocolitica isolates (n = 13) obtained previously from the same pork chain. A second PFGE analysis (NotI) confirmed the high degree of similarity among isolates obtained over time, demonstrating the persistence of an apparent clonal Y. enterocolitica bio-serotype 4/O:3 in this particular pork production chain in Brazil.

先前在巴西的猪肉生产链中鉴定到了小肠结肠炎耶尔森菌生物血清型4 / O：3，并且通过脉冲场凝胶电泳（PFGE，XbaI）获得的分离株具有较高的身份。在本研究中，两年后，从同一条猪肉生产链中又采集了147份猪样品（扁桃= 100，上颚= 30，头肉= 17），并且有14份（9.5％）猪小肠结肠炎耶尔森氏菌呈阳性。分离株（n = 24，每个阳性样品为1-2）为生物血清型4 / O：3，并带有毒力基因ail，inv，wbbU，virF，myfA，ystA，ymoA，hreP和sat以及与多药耐药相关的基因emrD，marC和yfhD。PFGE（XbaI）证明分离株之间没有差异（100％相似性），并且与以前从同一猪肉链获得的一些小肠结肠炎耶尔森氏菌分离株（n = 13）相同。PFGE的第二次分析（NotI）证实了随着时间的推移获得的分离株之间具有高度相似性，这表明在巴西这个特定的猪肉生产链中，明显存在的肠炎耶尔森氏菌生物血清型4 / O：3仍然存在。