Abstract The partitioning behavior of norfloxacin (more hydrophilic) and sodium diclofenac (more hydrophobic) was studied considering the use of aqueous two-phase systems.(ATPS) based in copolymers and dextran. For this research, the phase behavior of systems composed of water, a copolymer (Pluronic PE6400, Pluronic L35, Pluronic PE6800, PEG-ran-PPG monobutyl ether and PEG-ran-PPG) and dextran (Mw=40000 and 6000 g.mol−1) were investigated. The phase diagrams showed that by increasing the molecular weight and the copolymer PPG/PEG ratio, the biphasic region is enlarged. After defining the bionodal curves, a preliminary screening on the partition of two pharmaceutic ingredients (sodium diclofenac and norfloxacin) was carried. After selected the most appropriate ATPS for the partition of both drugs, namely systems based on diblock copolymers (poly(ethylene glycol)-poly(propylene glycol)) – dextran T6 and one random copolymer (poly(ethylene glycol)-ran-poly(propylene glycol)) – dextran T6, the effect of the copolymer concentration and different mixture points was assessed. In the end, the results showed that by increasing the copolymer concentration and tie-line length, the partition coefficient of the pharmaceutical ingredients increased in the block copolymer-based ATPS and decreased in systems containing the random copolymer.

中文翻译：

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双氯芬酸钠和诺氟沙星在基于共聚物和葡聚糖的水性两相体系中的分配研究

摘要 考虑使用基于共聚物和葡聚糖的水性两相系统（ATPS），研究了诺氟沙星（更亲水）和双氯芬酸钠（更疏水）的分配行为。在本研究中，由水、共聚物（Pluronic PE6400、Pluronic L35、Pluronic PE6800、PEG-ran-PPG 单丁醚和 PEG-ran-PPG）和葡聚糖（Mw=40000 和 6000 g.mol）组成的系统的相行为-1) 进行了调查。相图表明，通过增加分子量和共聚物 PPG/PEG 比率，双相区域扩大。在定义生物节曲线后，对两种药物成分（双氯芬酸钠和诺氟沙星）的分配进行了初步筛选。在为两种药物的分配选择最合适的ATPS后，即基于双嵌段共聚物（聚（乙二醇）-聚（丙二醇））-葡聚糖 T6 和一种无规共聚物（聚（乙二醇）-ran-聚（丙二醇））-葡聚糖 T6 的系统，共聚物的效果浓度和不同的混合点进行了评估。最后，结果表明，通过增加共聚物浓度和连接线长度，药物成分的分配系数在基于嵌段共聚物的 ATPS 中增加，在包含无规共聚物的体系中降低。