We examined Bartonella prevalence in 281 bat flies collected from 114 eastern bent-wing bats (Miniopterus fuliginosus) in Japan and phylogenetically analyzed with other bat fly and bat strains. The bat flies were identified as Penicilidia jenynsii (PJ; n = 45), Nycteribia allotopa (NA; n = 157), and novel Nycteribia species (NS; n = 79). Bartonella DNAs were detected in 31.7 % (89/281) of bat flies by PCR targeting the citrate synthase (gltA) gene. The prevalence of Bartonella DNA among the bat flies was 47.1 % (74/157) in NA, 15.2 % (12/79) in NS, and 6.7 % (3/45) in PJ. Bartonella bacteria were also isolated from two NA and one NS. A phylogenetic analysis of the gltA sequences revealed that bat fly-associated strains were classified into three lineages and the same lineages of Bartonella were commonly detected from both Nycteribia bat flies and Miniopterus bats. These results suggest that Nycteribia bat flies are potential vectors for transmitting Bartonella among Miniopterus bats.

我们检查了从日本的114个东部弯翅蝙蝠（Miniopterus fuliginosus）收集的281只蝙蝠中的巴尔通体流行情况，并用其他蝙蝠蝇和蝙蝠菌株进行了系统发育分析。球棒蝇被鉴定为Penicilidia jenynsii（PJ; N = 45），Nycteribia allotopa（NA; N = 157），和新颖的Nycteribia物种（NS; N = 79）。通过PCR靶向柠檬酸合酶（gltA）基因，在31.7％（89/281）的蝙蝠蝇中检测到巴尔通体DNA 。蝙蝠中Bartonella DNA的流行率在北美为47.1％（74/157），在NS为15.2％（12/79），在PJ为6.7％（3/45）。巴通体还从两个NA和一个NS中分离出细菌。所述的系统发生分析的gltA序列揭示了蝙蝠蝇相关菌株分类为三个谱系和相同谱系汉赛巴尔从两者都通常检测Nycteribia蝙蝠苍蝇和长翼蝙蝠。这些结果表明，Nycteribia蝠蝇是指为传输载体的潜力巴尔通体中折翼蝙蝠。