The etiology of neonatal diarrhea is multifactorial and remains one of the greatest health problems in sheep livestock farming. Faecal samples from 559 neonatal lambs aged less than 30 days from 30 sheepfolds located in the north-center region of Algeria were screened with pathogen-specific antigen ELISA for Cryptosporidium parvum, Escherichia coli K99, rotavirus, and coronavirus. Of the 559 lambs, 312 (58.81 %), 155 (27.72 %), 72 (12.88 %) and 20 (3.57 %) were positives for C. parvum, E. coli K99, rotavirus and coronavirus antigens, respectively. The prevalence of C. parvum was the highest (p < 0.0001). C. parvum, E. coli K99, rotavirus and coronavirus were observed in 23 (76.66 %), 17 (56.66 %), 9 (30 %) and 3 (10 %) sheepfolds, respectively. Compared to age, the prevalence of C. parvum was highest during the second and third week of age (p < 0.001). In contrast, other pathogens were found to be more frequent in lambs aged ≤7 days (p < 0.001). The number of lambs with diarrhea was 280 (50.09 %) of which 280 (100 %), 127 (45.35 %), 52 (18.57 %) and 10 (3.57 %) were found to be infected with C. parvum, E. coli K99, rotavirus and coronavirus, respectively (p < 0.0001). In various combinations, mixed infections were detected only with C. parvum. This is the first report of C. parvum, E. coli K99, rotavirus, and coronavirus in ≤30-days old neonatal lambs in Algeria. Special attention should be given to the first colostrum feeding, hygiene of the farm, prevention and control measures for a better prevention of neonatal diarrhea in lambs.

新生儿腹泻的病因是多方面的，并且仍然是绵羊畜牧业中最大的健康问题之一。用病原体特异性抗原ELISA筛选了来自阿尔及利亚北中部地区的559只新生羔羊的粪便样本，这些羊羔的年龄不到30天，该羊群的病原体特异性抗原ELISA为小隐隐孢子虫，大肠杆菌K99，轮状病毒和冠状病毒。559只羊羔，312（58.81％），155（27.72％），72（12.88％）和20（3.57％）是作阳性微小隐孢子虫，大肠杆菌分别K99，轮状病毒和冠状病毒抗原，。微小梭状芽胞杆菌的患病率最高（p <0.0001）。小肠梭菌，大肠杆菌分别在23（76.66％），17（56.66％），9（30％）和3（10％）的羊圈中观察到K99，轮状病毒和冠状病毒。与年龄相比，小腿梭状芽胞杆菌的患病率在年龄的第二周和第三周最高（p <0.001）。相反，发现其他病原体在≤7天的羔羊中更常见（p <0.001）。腹泻的羔羊数量为280（50.09％），其中280（100％），127（45.35％），52（18.57％）和10（3.57％）被发现感染了C. parvum，大肠杆菌。分别是K99，轮状病毒和冠状病毒（p <0.0001）。在各种组合中，仅用小球藻检测到混合感染。这是首次报道微小隐孢子虫，大肠杆菌阿尔及利亚≤30天大的新生羔羊中的K99，轮状病毒和冠状病毒。应特别注意初乳喂养，农场卫生，预防和控制措施，以更好地预防羊羔的新生儿腹泻。