The antidepressant, venlafaxine (VFX), and climate change stressors, such as increased water temperature and decreased dissolved oxygen, are current threats to aquatic environments. This study aimed to determine how microRNAs (miRNAs) and predicted targeted transcripts were altered in livers of zebrafish exposed to these stressors, and livers of their un-exposed F₁ and F₂ offspring. Following a 21 day exposure to multiple stressors (1 μg/L VFX, +5 °C ambient, 50% O₂), then a subsequent 21 day recovery, relative abundances of cyp3a65, hsp70, hsp90, and ppargc1a and miRNAs predicted to target them (miR-142a, miR-16c, miR-181c, and miR-129, respectively) were measured in the liver via quantitative PCR (RT-qPCR). There were significant decreases in miR-142a in the exposed F₀ generation and the exposed F₁ generation. While there were no changes detected in cyp3a65 relative abundance, there was a significant inverse relationship between cyp3a65 and miR-142a. Hsp70 expression significantly increased in the F₁ generation, which persisted to the F₂ generation and the relative abundance of hsp90 significantly increased in all generations. There was a significant reduction in miR-181c in the F₁ generation, but there was no significant relationship between miR-181c and hsp90. Finally, there was a significant decrease in ppargc1a relative abundance in the F₁ generation which was associated with an increase in miR-129. Combined, these results suggest that parental exposure to multiple, environmentally relevant stressors can confer transcriptional and epigenetic responses in the F₁ and F₂ generations, although identifying which stressor is a driving force becomes unclear.

抗抑郁药文拉法辛（VFX）和气候变化压力源，例如水温升高和溶解氧降低，是目前对水生环境的威胁。这项研究旨在确定暴露于这些应激源的斑马鱼肝脏及其未暴露的F ₁和F ₂后代肝脏中的microRNA（miRNA）和预测的目标转录本是如何改变的。在21天暴露于多种应激源（1μg/ L VFX，+ 5°C环境，50％O ₂）之后，随后的21天恢复，cyp3a65，hsp70，hsp90和ppargc1a的相对丰度通过定量PCR（RT-qPCR）在肝脏中测量了预期靶向它们的miRNA（分别为miR-142a，miR-16c，miR-181c和miR-129）。在暴露的F ₀世代和暴露的F ₁世代中，miR-142a显着降低。虽然cyp3a65相对丰度未检测到变化，但cyp3a65与miR-142a之间存在显着的逆相关。Hsp70表达在F ₁世代中显着增加，并持续到F ₂世代，并且hsp90的相对丰度在所有世代中均显着增加。F中miR-181c显着降低₁代，但miR-181c和hsp90之间没有显着关系。最后，F ₁代中ppargc1a的相对丰度显着下降，这与miR-129的增加有关。综合起来，这些结果表明，父母对多种与环境有关的应激源的暴露可以在F ₁和F ₂世代中赋予转录和表观遗传反应，尽管目前尚不清楚哪种应激源是驱动力。