We evaluate the effect of cationic nanocapsules containing dihydromyricetin (DMY) flavonoid for safe topical use in photoprotection against UV-induced DNA damage. The stability was investigated for feasibility to produce hydrogels containing cationic nanocapsules of the flavonoid DMY (NC-DMY) for 90 days under three different storage conditions (4 ± 2 °C, 25 ± 2 °C, and 40 ± 2 °C), as well as evaluation of skin permeation and its cytotoxicity in skin cell lines. The physicochemical and rheological characteristics were maintained during the analysis period under the different aforementioned conditions. However, at 25 °C and 40 °C, the formulations indicated yellowish coloration and DMY content reduction. Therefore, the ideal storage condition of 4 °C was adopted. DMY remained in the stratum corneum and the uppermost layers of the skin. Regarding safety, all formulations demonstrated to be safe for topical application. NC-DMY exhibited a 50% Solar Protection Factor (SPF-DNA) against DNA damage caused by UVB radiation and demonstrated 99.9% protection against DNA lesion induction. These findings establish a promising formulation containing nanoencapsulated DMY flavonoids with a photoprotective and antioxidant potential of eliminating reactive oxygen species formed by solar radiation.

我们评估了含有二氢杨梅酮（DMY）类黄酮的阳离子纳米胶囊对于安全局部使用的抗紫外线引起的DNA损伤的光保护作用。研究了在三种不同的储存条件下（4±2°C，25±2°C和40±2°C）90天生产含类黄酮DMY（NC-DMY）阳离子纳米胶囊的水凝胶的可行性的稳定性，以及评估皮肤渗透性及其在皮肤细胞系中的细胞毒性。在分析期间，在上述不同条件下保持了理化和流变特性。然而，在25℃和40℃下，制剂显示出淡黄色和DMY含量降低。因此，采用了4°C的理想存储条件。DMY残留在角质层和皮肤的最上层。关于安全性，所有制剂均证明对局部应用安全。NC-DMY对UVB辐射引起的DNA损伤表现出50％的太阳保护因子（SPF-DNA），对DNA损伤的诱导显示99.9％的保护作用。这些发现建立了一种有前途的配方，该配方包含纳米封装的DMY类黄酮，具有消除由太阳辐射形成的活性氧的光保护和抗氧化潜力。