Multifunctional enzymatic nanomaterials (nanozymes) with good biocompatibility, strong enzymatic activity, and substrate specificity are the desired alternatives to natural enzymes. Here, the design of a peroxidase mimetic nanozyme capable of detecting and depleting glutathione (GSH) in the bacterial cells is presented. The nanozyme palladium-iron nanostructure decorated graphdiyne nanosheet (PdFe/GDY) was prepared by a facile hydrothermal approach. It can efficiently catalyze the decomposition of H₂O₂ to produce •OH by a peroxidase-like mechanism. Surprisingly, the PdFe/GDY nanozymes can detect GSH in bacteria with excellent specificity and ultra-low detection limit of 24.45 nM. Moreover, the PdFe/GDY nanozymes can consume GSH for bacterial disinfection with killing efficacy >99.99%, promote wound healing and do not cause significant toxicity in vitro and in vivo. To our knowledge, this is the first example of a GDY-based nanozyme biosensor and antibacterial agent. Collectively, this work would be indicative to construct GDY-based nanozymes in clinical diagnosis and sterilization.

具有良好的生物相容性，强大的酶活性和底物特异性的多功能酶纳米材料（纳米酶）是天然酶的理想替代品。在此，提出了一种能够检测和消耗细菌细胞中谷胱甘肽（GSH）的过氧化物酶模拟纳米酶的设计。纳米酶钯铁纳米结构修饰的石墨二炔纳米片（PdFe / GDY）是通过简便的水热法制备的。它可以有效地催化H ₂ O ₂的分解通过类似过氧化物酶的机制生成•OH。令人惊讶的是，PdFe / GDY纳米酶可以以优异的特异性和24.45 nM的超低检测限检测细菌中的GSH。此外，PDFE / GDY nanozymes可以消耗GSH用于细菌消毒与杀灭功效> 99.99％，促进伤口愈合，并且不会导致显著毒性在体外和体内。据我们所知，这是基于GDY的纳米酶生物传感器和抗菌剂的第一个实例。总的来说，这项工作将指示在临床诊断和灭菌中构建基于GDY的纳米酶。