In this study, the melanoma targeting property of ⁶⁷Ga-NODAGA-GGNle-CycMSH_hex {1,4,7-triazacyclononane,1-gluteric acid-4,7-acetic acid-GlyGlyNle-c[Asp-His-D-Phe-Arg-Trp-Lys]-CONH₂} was determined on B16/F10 melanoma-bearing C57 mice to demonstrate the feasibility of NODAGA as a radiometal chelator for facile room temperature radiolabeling of NODAGA-GGNle-CycMSH_hex. The IC₅₀ value of NODAGA-GGNle-CycMSH_hex was 0.87 ± 0.12 nM on B16/F10 melanoma cells. ⁶⁷Ga-NODAGA-GGNle-CycMSH_hex was readily prepared at room temperature with greater than 98% radiolabeling yield and displayed MC1R-specific binding on B16/F10 melanoma cells. The B16/F10 melanoma uptake of ⁶⁷Ga-NODAGA-GGNle-CycMSH_hex was 10.31 ± 0.78, 14.96 ± 1.34, 13.7 ± 3.33 and 10.4 ± 2.2% ID/g at 0.5, 2, 4 and 24 h post-injection, respectively. Approximately 85% of the injected dose was cleared out the body via urinary system at 2 h post-injection. ⁶⁷Ga-NODAGA-GGNle-CycMSH_hex showed high tumor/blood, tumor/muscle and tumor/skin uptake ratios after 2 h post-injection. Overall, ⁶⁷Ga-NODAGA-GGNle-CycMSH_hex could be easily prepared at room temperature and exhibited favorable melanoma targeting property, suggesting the potential use of NODAGA as a radiometal chelator for facile room temperature radiolabeling of α-MSH peptides.