The integration of chemical catalyst and biocatalyst is of great interest due to the accompanying high designability of chemo-enzymatic cascade reactions for sustainable catalytic processes in chemical engineering. Here, a facile strategy is proposed by preparation of a copper ions coordinated cytidine monophosphate (CMP) encapsulated lipase composite via in-situ assembly in aqueous solution which followed by reduction to obtain an enzyme-copper hybrid catalyst. The as-synthesized enzyme-copper nucleotide composite exhibited both high enzymatic activity (6.05 U/mg) and copper-catalytic activity (with reaction rate constant of 8.85 min⁻¹) in the chemo-enzymatic cascade reaction for hydrolysis of p-nitrophenyl butyrate (p-NPB) and generation of p-aminophenol (p-AP), which is a pharmaceutical intermediate for production of analgesics and antipyretics. This study thus sheds light on fabrication of hybrid catalyst under facile conditions, exhibiting potential in development of biosensors, industrial catalysis and biomanufacturing.

化学催化剂和生物催化剂的集成引起人们极大的兴趣，这是因为化学酶联反应的高度可设计性可用于化学工程中的可持续催化过程。在此，通过在水溶液中原位组装制备铜离子配位的胞苷单磷酸（CMP）包封的脂肪酶复合物，提出了一种简便的策略，然后还原以获得酶-铜杂化催化剂。合成的酶-铜核苷酸复合物在化学酶联级联反应中水解对硝基苯基丁酸酯时，具有很高的酶活性（6.05 U / mg）和铜催化活性（反应速率常数为8.85 min ^-1）。（p-NPB）和对氨基苯酚（p -AP）的产生，这是用于生产止痛药和退烧药的药物中间体。因此，这项研究为在容易的条件下制备杂化催化剂提供了启示，在生物传感器的开发，工业催化和生物制造方面显示出潜力。