At the forefront of synthetic endeavors in the pharmaceutical industry, including drug discovery and high-throughput screening, timelines are tight and large quantities of pure chemical targets are rarely available. In this regard, the development of novel and increasingly challenging chemistries requires a commensurate level of innovation to develop reliable analytical assays and purification workflows with rapid turnaround that enables accelerated pharmacological evaluation. A small-scale automation platform enabling high-throughput analysis and purification to streamline the selection of candidate leads would be a transformative advance. Herein, we introduce an automation-friendly solid-phase extraction-matrix-assisted laser desorption/ionization (SPE-MALDI) platform applied to the high-throughput purification and analysis of peptide libraries. This advance enabled us to purify peptides from microgram levels in less than a day with results comparable to traditional high-performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-MS).

中文翻译：

---

超高通量 SPE-MALDI 工作流程：高效纯化和筛选肽库的蓝图

在制药行业合成努力的最前沿，包括药物发现和高通量筛选，时间紧迫，而且很少有大量纯化学靶点可用。在这方面，新的和越来越具有挑战性的化学物质的开发需要相应水平的创新，以开发可靠的分析检测和纯化工作流程，并具有快速周转的能力，从而能够加速药理学评估。一个能够进行高通量分析和纯化以简化候选线索选择的小型自动化平台将是一个变革性的进步。在此，我们介绍了一种自动化友好的固相萃取-基质辅助激光解吸/电离 (SPE-MALDI) 平台，适用于肽库的高通量纯化和分析。