Mosquitoes are commonly identified to species level using morphological traits, but complementary methods for identification are often necessary when specimens are collected as immature stages, stored inadequately, or when delineation of species complexes is problematic. DNA-barcoding using the mitochondrial cytochrome c oxidase subunit 1 (COI) gene is one such tool used for the morphological identification of species. A comprehensive entomological survey of mosquito species in Mexico State identified by COI DNA barcoding and morphology is documented in this paper. Specimens were collected from all the physiographic provinces in Mexico State between 2017 and 2019. Overall, 2,218 specimens were collected from 157 localities representing both subfamilies Anophelinae and Culicinae. A species checklist that consists of 6 tribes, 10 genera, 20 subgenera, and 51 species, 35 of which are new records for Mexico State, is provided. Three hundred and forty-two COI sequences of 46 species were analysed. Mean intraspecific and interspecific distances ranged between 0% to 3.9% and from 1.2% to 25.3%, respectively. All species groups were supported by high bootstraps values in a Neighbour-Joining analysis, and new COI sequences were generated for eight species: Aedes chionotum Zavortink, Ae. vargasi Schick, Ae. gabriel Schick, Ae. guerrero Berlin, Ae. ramirezi Vargas and Downs, Haemagogus mesodentatus Komp and Kumm, Culex restrictor Dyar and Knab, and Uranotaenia geometrica Theobald. This study provides a detailed inventory of the Culicidae from Mexico State and discusses the utility of DNA barcoding as a complementary tool for accurate mosquito species identification in Mexico.

通常使用形态学特征将蚊子识别到物种水平，但是当标本采集为未成熟阶段，存储不充分或在描述物种复合体时遇到问题时，通常需要使用互补的识别方法。使用线粒体细胞色素C氧化酶亚基1（COI）基因进行DNA条形码编码是一种用于物种形态鉴定的工具。COI确定的墨西哥州蚊子种类的综合昆虫学调查DNA条形码和形态学在本文中有记载。在2017年至2019年之间，从墨西哥州的所有地理省份收集了标本。总体上，从157个地区（分别代表按蚊亚科和库里科亚）收集了2218个标本。提供了一个物种清单，该清单包括6个部落，10个属，20个亚属和51个物种，其中35个是墨西哥州的新记录。三百42 COI的46种序列进行分析。种内和种间平均距离分别在0％至3.9％和1.2％至25.3％之间。在Neighbour-Joining分析中，所有物种组都得到了高引导值的支持，并且为八个物种生成了新的COI序列：Aedes chionotum扎沃廷克，爱。vargasi希克，阂。加布里埃尔·史基克（Ae。）游击队柏林，ae。ramirezi Vargas和Downs，Haemagogus mesodentatus Komp和Kumm，Culex限流器Dyar和Knab，以及Uranotaenia geometrica Theobald。这项研究提供了来自墨西哥州的ida科的详细清单，并讨论了DNA条形码作为在墨西哥准确识别蚊子种类的辅助工具的实用性。