When nanoparticles were introduced into the biological media, the protein corona would be formed, which endowed the nanoparticles with new bio-identities. Thus, controlling protein corona formation is critical to in vivo therapeutic effect. Controlling the particle size is the most feasible method during design, and the influence of media pH which varies with disease condition is quite important. The impact of particle size and pH on bovine serum albumin (BSA) corona formation of solid lipid nanoparticles (SLNs) was studied here. The BSA corona formation of SLNs with increasing particle size (120–480 nm) in pH 6.0 and 7.4 was investigated. Multiple techniques were employed for visualization study, conformational structure study and mechanism study, etc. “BSA corona-caused aggregation” of SLN2‒3 was revealed in pH 6.0 while the dispersed state of SLNs was maintained in pH 7.4, which significantly affected the secondary structure of BSA and cell uptake of SLNs. The main interaction was driven by van der Waals force plus hydrogen bonding in pH 7.4, while by electrostatic attraction in pH 6.0, and size-dependent adsorption was confirmed. This study provides a systematic insight to the understanding of protein corona formation of SLNs.

当纳米颗粒被引入生物介质中时，会形成蛋白质冠，从而赋予纳米颗粒新的生物特性。因此，控制蛋白质冠的形成对于体内治疗效果至关重要。设计时控制粒径是最可行的方法，而随病况变化的介质pH值的影响相当重要。本文研究了粒径和 pH 对固体脂质纳米颗粒 (SLN) 牛血清白蛋白 (BSA) 电晕形成的影响。研究了在 pH 6.0 和 7.4 条件下随着粒径增加 (120-480 nm) 的 SLN 的 BSA 电晕形成。采用多种技术进行可视化研究、构象结构研究和机制研究等。在pH 6.0时，SLN2-3出现“BSA电晕引起的聚集”，而在pH 7.4时，SLN仍保持分散状态，这显着影响了次级BSA 的结构和 SLN 的细胞摄取。在pH 7.4 中，主要相互作用是由范德华力加氢键驱动的，而在pH 6.0 中是由静电引力驱动的，并且证实了尺寸依赖性吸附。这项研究为理解 SLN 的蛋白冠形成提供了系统的见解。