This is the first work focused on glycoprofiling of whole N- and O- glycome using lectins in an array format applied for analysis of serum samples from healthy individuals, benign prostate hyperplasia (BPH) patients, and prostate cancer (PCa) patients. Lectin microarray was prepared using traditional lectins with the incorporation of 2 recombinant bacterial lectins and 3 human lectins (17 lectins in total). Clinical validation of glycans as biomarkers was done in two studies: discrimination of healthy individuals with BPH patients vs. PCa patients (C vs. PCa) and discrimination of healthy individuals vs. BPH and PCa patients (H vs. PCond). Single lectins (17 lectins) and a combination of two lectins (136 binary lectin combinations) were applied in the clinical validation of glycan biomarkers providing 153 AUC values from ROC curves for both studies (C vs. PCa and H vs. PCond). Potential N- and O-glycans as biomarkers were identified and possible carriers of these glycans are shortly discussed.

这是第一项专注于使用阵列形式的凝集素对整个N - 和O - 糖组进行糖谱分析的工作，用于分析来自健康个体、良性前列腺增生 (BPH) 患者和前列腺癌 (PCa) 患者的血清样本。使用传统凝集素制备凝集素微阵列，其中掺入了 2 种重组细菌凝集素和 3 种人类凝集素（总共 17 种凝集素）。在两项研究中对聚糖作为生物标志物进行了临床验证：区分患有 BPH 患者的健康个体与 PCa 患者（C 与 PCa）以及区分健康个体与 BPH 和 PCa 患者（H 与 PCond）。将单一凝集素（17 种凝集素）和两种凝集素的组合（136 种二元凝集素组合）应用于聚糖生物标志物的临床验证，这两项研究（C 与 PCa 和 H 与 PCond）的 ROC 曲线提供了 153 个 AUC 值。确定了潜在的N - 和O -聚糖作为生物标志物，并简要讨论了这些聚糖的可能载体。