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Simultaneous qualitative characterization of four herbs in Weikangling capsules by a validated high-performance thin-layer chromatography method
JPC - Journal of Planar Chromatography - Modern TLC ( IF 1.1 ) Pub Date : 2020-10-30 , DOI: 10.1007/s00764-020-00060-x
Feng Liu , Mengyue Wang , Xiaobo Li

The rapid and handy high-performance thin-layer chromatography (HPTLC) method has been widely adopted to qualitatively identify herbs or medicinal materials from Chinese patent medicine. Weikangling capsules, as one of the traditional Chinese patent medicines for treating chronic gastritis, consist of Paeoniae Radix Alba, Glycyrrhizae Radix et Rhizoma, Bletillae Rhizoma, Notoginseng Radix et Rhizoma, Poria, Corydalis Rhizoma, Sepiae Endoconcha, and Belladonnae Extractum. However, there has been no effective method to identify both monarch and minister drugs in the prescription simultaneously so far. We report in this paper a new HPTLC method for the simultaneous qualitative identification of four herbs in Weikangling capsules using the lower layer of the chloroform‒methanol‒water (14:5:1, V/V) mixture as the mobile phase. Importantly, the method achieved “one-plate multi-drug” and could identify the monarch drugs (Paeoniae Radix Alba and Glycyrrhizae Radix et Rhizoma) and the minister drugs (Bletillae Rhizoma and Notoginseng Radix et Rhizoma) at the same time, which could better reflect the effect of emolliating the liver and harmonizing the stomach, dissipating stasis, and stopping bleeding.



中文翻译:

经验证的高效薄层色谱法同时对微康灵胶囊中的四种草药进行定性表征

快速便捷的高效薄层色谱法(HPTLC)已被广泛用于定性鉴定中成药中的草药或药用材料。卫康灵胶囊是治疗慢性胃炎的传统中成药之一,由e药白Alba,甘草和大黄,牛肝菌,三七,大黄,、 Por,延胡索,乌贼仁和颠茄提取物组成。但是,到目前为止,还没有有效的方法可以同时识别处方中的帝王药和部长药。我们在本文中报告了一种新的HPTLC方法,该方法同时使用氯仿‒甲醇‒水的下层(14:5:1,V / V)同时定性鉴定了维康灵胶囊中的四种草药)混合物作为流动相。重要的是,该方法实现了“单板多药”,并且可以同时识别帝王药(Pa药丹参和甘草Rad药等)和大臣药(白let药和三七药等)。反映了润肝,调和胃,散瘀,止血的功效。

更新日期:2020-10-30
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