C₆₀ is a potent antioxidant that has been reported to substantially extend the lifespan of rodents when formulated in olive oil (C₆₀-OO) or extra virgin olive oil (C60-EVOO). Despite there being no regulated form of C₆₀-OO, people have begun obtaining it from online sources and dosing it to themselves or their pets, presumably with the assumption of safety and efficacy. In this study, we obtain C₆₀-OO from a sample of online vendors, and find marked discrepancies in appearance, impurity profile, concentration, and activity relative to pristine C₆₀-OO formulated in-house. We additionally find that pristine C₆₀-OO causes no acute toxicity in a rodent model but does form toxic species that can cause significant morbidity and mortality in mice in under 2 weeks when exposed to light levels consistent with ambient light. Intraperitoneal injections of C₆₀-OO did not affect the lifespan of CB6F1 female mice. Finally, we conduct a lifespan and health span study in males and females C57BL/6 J mice comparing oral treatment with pristine C₆₀-EVOO and EVOO alone versus untreated controls. We failed to observe significant lifespan and health span benefits of C₆₀-EVOO or EVOO supplementation compared to untreated controls, both starting the treatment in adult or old age. Our results call into question the biological benefit of C₆₀-OO in aging.

C ₆₀是一种有效的抗氧化剂，据报道，当配制在橄榄油 (C ₆₀ -OO) 或特级初榨橄榄油 (C60-EVOO) 中时，可以显着延长啮齿动物的寿命。_{尽管 C 60} -OO没有受监管的形式，但人们已经开始从在线来源获取它，并给自己或宠物服用，大概是在安全性和有效性的假设下。在这项研究中，我们从在线供应商的样本中获得了 C ₆₀ -OO，并发现与内部配制的原始 C _{60 -OO 在外观、杂质分布、浓度和活性方面存在显着差异。}我们还发现，原始的 C ₆₀ -OO 在啮齿动物模型中不会引起急性毒性，但确实会形成有毒物质，当暴露于与环境光一致的光水平时，可在不到 2 周内导致小鼠显着发病和死亡。腹腔注射C ₆₀ -OO不影响CB6F1雌性小鼠的寿命。最后，我们对雄性和雌性 C57BL/6 J 小鼠进行了一项寿命和健康寿命研究，将口服原始 C ₆₀ -EVOO 和单独的 EVOO 治疗与未治疗的对照组进行比较。与未经治疗的对照相比，我们未能观察到 C ₆₀ -EVOO 或 EVOO 补充剂对寿命和健康寿命的显着益处，两者均在成年或老年开始治疗。_{我们的结果对 C 60} -OO 在衰老中的生物学益处提出了质疑。