Abstract

Gibberellin signaling pathway genes encoding the DELLA protein and gibberellin receptor GID1 were sequenced in several varieties (Alpha, Valdai, Orlovskaya 9, Pracha) and one line of rye (EM-1) using next generation methods. The revealed multiple alleles of these genes differ mainly in single-nucleotide polymorphisms and less frequently in insertions and deletions. Most of the detected mutations turned out to be synonymous or located in the noncoding regions of the genes. Changes in the amino acid sequences of proteins associated with other mutations are probably functionally neutral. Mutations similar to wheat reduced-height gibberellin-insensitive alleles were not detected.

中文翻译：

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栽培黑麦中赤霉素信号通路基因的分子分析

摘要

使用下一代方法，对编码DELLA蛋白和赤霉素受体GID1的赤霉素信号传导途径基因进行了测序（包括Alpha，Valdai，Orlovskaya 9，Pracha）和多个黑麦（EM-1）品种。这些基因的揭示的多个等位基因主要在单核苷酸多态性方面不同，而在插入和缺失方面则较不频繁。大部分检测到的突变被证明是同义的或位于基因的非编码区。与其他突变相关的蛋白质的氨基酸序列变化可能在功能上是中性的。未检测到类似于小麦减低赤霉素不敏感等位基因的突变。