Abstract—

A complex of proteolytic enzymes with a specific plasmin-like activity of at least 890 E_pNA/mg and a specific protein C activator activity of at least 130 E_pNA/mg was isolated from the culture fluid of micromycete Aspergillus ochraceus L-1 by salting out with ammonium sulfate followed by dialysis and depigmentation to phenol-aniline formaldehyde resin at a pH of 8.2. Comparison of the obtained proteinase preparation with caseinolytic, fibrinolytic, fibrinogenolytic, and plasmin-like activities with commercial analogues terrilytin, trypsin, and streptokinase showed its promise for use as a means for thinning purulent burn wounds and fibrin clots. The resulting proteinase complex had high storage stability at low temperatures for up to 9 months.

中文翻译：

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曲霉L-1蛋白酶复合物的生产和稳定性及其纤溶和抗凝活性

摘要-

通过盐析从微霉菌L曲霉L-1的培养液中分离出具有至少890 E _pNA / mg的特定纤溶酶样活性和至少130 E _pNA / mg的特定蛋白C活化剂活性的蛋白水解酶复合物。用硫酸铵除去，然后在8.2的pH下渗析和脱色为苯酚-苯胺甲醛树脂。将获得的蛋白酶制剂与酪蛋白水解酶，纤维蛋白溶解酶，纤维蛋白原分解酶和纤溶酶样活性与市售类似物terlylytin，胰蛋白酶和链激酶比较，表明其有望用作减轻化脓性烧伤和纤维蛋白凝块的手段。所得的蛋白酶复合物在低温下具有长达9个月的高存储稳定性。