The substrate efficiency of modified derivatives of 2'-deoxyuridine-(mod-dUTP) and 2'-deoxycytidine-5'-triphosphates (mod-dCTP) was studied in the regime of simultaneous incorporation using the primer elongation reaction (PEX) and PCR with complete replacement of natural deoxytimidine (dT) and deoxycytidine (dC). Polymerases with missing 3'–5' exonuclease activity were used: Taq and Vent (exo-), as well as natural and constructed DNA templates. DNA fragments modified simultaneously by two types of nucleotides with similar and different functional groups were obtained. Research is important for obtaining aptamers with extended properties.

中文翻译：

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使用 PEX 和 PCR 在生长的 DNA 链中同时掺入修饰的 dU 和 dC 衍生物

使用引物延伸反应 (PEX) 和 PCR 在同时掺入的情况下研究了 2'-脱氧尿苷-(mod-dUTP) 和 2'-脱氧胞苷-5'-三磷酸 (mod-dCTP) 的修饰衍生物的底物效率完全替代天然脱氧嘧啶 (dT) 和脱氧胞苷 (dC)。使用缺少 3'–5' 核酸外切酶活性的聚合酶：Taq 和 Vent (exo-)，以及天然和构建的 DNA 模板。获得了同时被具有相似和不同功能基团的两种核苷酸修饰的DNA片段。研究对于获得具有扩展特性的适体很重要。