Abstract

Shaggy-related protein kinase (SK) plays important roles in the plant growth development, signal transduction, abiotic stress and biotic stress and substance metabolism regulation. In the present paper, a cDNA sequence encoding MmSK (GenBank accession No: KY348867) was cloned from the leaves of mulberry based on mulberry expressed sequence tags (EST) and homologous cloning technology using RT-PCR, which was 1705 bp in length with a full open reading frame (ORF) of 1236 bp encoding a protein of 411 amino acids. The estimated molecular weight and isoelectric point (pI) of the putative protein were 46.55 kDa and 8.61, respectively. Conservation domain structure analysis indicated that MmSK protein had typical structure of the protein kinase domain and belonged to GSK3/shaggy protein kinase family. Multiple sequence alignment and phylogenetic analysis showed that the homology between the amino acid sequences encoded by the MmSK gene and various species was more than 89%. Quantitative real-time PCR (qRT-PCR) analysis revealed that MmSK was expressed in all the tested tissues including leaf, bud, fruit, stem, phloem and xylem of the mulberry with the highest expression in the phloem. The expression level of the mRNA has changed significantly under salt, drought, cold and ABA stress treatments compared to the normal growth environment. Overall, these results showed a better understanding of the molecular basis for the signal transduction mechanism during the stress responses in mulberry trees.

中文翻译：

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桑树破骨相关激酶（MmSK）基因的分子鉴定和表达模式。

摘要

与毛茸茸相关的蛋白激酶（SK）在植物生长发育，信号转导，非生物胁迫以及生物胁迫和物质代谢调节中起重要作用。在本文中，编码MmSK的cDNA序列基于桑树表达序列标签（EST）和使用RT-PCR的同源克隆技术，从桑树的叶片中克隆了GenBank登录号（KY348867），全长1705 bp，全开放阅读框（ORF）为1236 bp编码411个氨基酸的蛋白质。推定蛋白质的估计分子量和等电点（pI）分别为46.55 kDa和8.61。保守结构域结构分析表明，MmSK蛋白具有典型的蛋白激酶结构域结构，属于GSK3 / shaggy蛋白激酶家族。多重序列比对和系统进化分析表明，MmSK基因编码的氨基酸序列与不同物种的同源性超过89％。实时定量PCR（qRT-PCR）分析显示MmSK在所有测试组织中表达，包括桑叶的叶，芽，果实，茎，韧皮部和木质部，在韧皮部中表达最高。与正常生长环境相比，在盐，干旱，冷和ABA胁迫处理下，mRNA的表达水平发生了显着变化。总体而言，这些结果显示了对桑树应激反应期间信号转导机制的分子基础的更好理解。