Plants of beggarticks (Bidens pilosa L.), honeyweed (Leonurus sibiricus L.), and citrus (Citrus ssp.) trees exhibiting phyllody and virescence, leaf distortion, and yellow shoots symptoms, respectively, were found in orchards at the state of Sao Paulo. Based on PCR assays, computer-simulated RFLP, and phylogenetic analyses, it was confirmed that these plants were infected with a phytoplasma of the subgroup 16SrIII-X, belonging to the ‘Candidatus Phytoplasma pruni’ species. This finding suggested that beggartick and honeyweed plants may be possible reservoirs of 16SrIII-X phytoplasma to citrus tree. The phytoplasma identified in this study presented perfect identity with the phytoplasma previously reported in association with citrus plants harboring HLB-like symptoms, in Brazil.

中文翻译：

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与巴西柑橘中的 HLB 样症状相关的 'Candidatus Phytoplasma pruni' 相关菌株 (16SrIII-X) 的潜在宿主

在 Sao 州的果园中发现了分别表现出叶状和绿化、叶片变形和黄芽症状的 beggarticks (Bidens pilosa L.)、蜜草 (Leonurus sibiricus L.) 和柑橘 (Citrus ssp.) 树的植物保罗。基于 PCR 分析、计算机模拟 RFLP 和系统发育分析，证实这些植物感染了属于 'Candidatus Phytoplasma pruni' 物种的 16SrIII-X 亚群的植原体。这一发现表明，beggartick 和蜜草植物可能是柑橘树 16SrIII-X 植原体的储存库。本研究中鉴定的植原体与之前在巴西报道的与具有 HLB 样症状的柑橘植物相关的植原体完全相同。