The Journal of Membrane Biology ( IF 2.3 ) Pub Date : 2020-10-22 , DOI: 10.1007/s00232-020-00144-z N A Ghazaryan 1 , A Kishmiryan 1 , N M Ayvazyan 1
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Abstract
It is known that snake venoms are a complex of enzymes and proteins and the interaction of different venom components with the membranes could be significantly enhanced in course of their action in an orchestra. The aim of the proposed investigation is to obtain detailed information about the mechanism and topology of two snake venom PLA2 isoforms from the Macrovipera lebetina obtusa venom in the membrane-binding process. We investigated the impact of the interaction on the properties of the model membrane (namely, GUVs and erythrocytes ghost) for each of these isoforms, as well as their synergetic action if they act simultaneously. The 6-lauroyl-2-dimethylaminonaphthalene and 6-propionyl-2-dimethylaminonaphthalene fluorescence probes were used to allow us to determine the membrane polarity more accurately via a generalized polarization function. Our results show that two types of PLA2 bring viscosity reduction in GUVs membrane and the effect became more potent when these PLA2 acts together. Intriguingly, we have not observed any significant difference in the case of the erythrocytes ghost membrane.
Graphic Abstract
中文翻译：
Macrovipera lebetina obtusa 毒液的两种Pla 2 组分对模型膜的促膜作用的协同作用
摘要
众所周知，蛇毒是酶和蛋白质的复合物，不同毒液成分与膜的相互作用在管弦乐队中的作用过程中可以显着增强。拟议调查的目的是从Macrovipera lebetina obtusa 中获取有关两种蛇毒 PLA2 同种型的机制和拓扑结构的详细信息毒液在膜结合过程中。我们研究了相互作用对每个这些同种型的模型膜（即 GUV 和红细胞鬼影）特性的影响，以及它们同时作用时的协同作用。6-月桂酰基-2-二甲基氨基萘和6-丙酰基-2-二甲基氨基萘荧光探针用于通过广义偏振函数更准确地确定膜极性。我们的结果表明，两种类型的 PLA2 使 GUVs 膜的粘度降低，当这些 PLA2 一起作用时，效果变得更加有效。有趣的是，我们没有观察到红细胞影膜的任何显着差异。
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