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Diploid Potato Germplasm with Resistance to Dickeya solani
Potato Research ( IF 2.3 ) Pub Date : 2020-10-24 , DOI: 10.1007/s11540-020-09482-w
Renata Lebecka , Iwona Wasilewicz-Flis , Dariusz Mańkowski

Potato growers suffer losses due to potato soft rot and blackleg caused by pectinolytic bacteria of different species. These bacteria cause yield loss during cultivation and storage, and can be transmitted to the next generations through seed tubers. The prevalence of Pectobacterium atrosepticum has decreased over the last 20 years in Europe in favour of Dickeya solani and P. carotovorum subsp. brasiliense. Twenty-six diploid interspecific Solanum hybrids, previously selected for tuber resistance to P. atrosepticum, were screened for resistance to a highly aggressive D. solani strain in terms of tuber and blackleg reaction. The bacterial strain used for inoculation was characterised by a relatively high optimal growth temperature in vitro, ranging from 33 to 35 °C. Twenty-four diploid clones did not differ in terms of tuber reaction to inoculation with this strain of bacteria in comparison with the clone USA 249, which is the somatic hybrid of S. brevidens (+) S. tuberosum with proven resistance to Pectobacterium. Seventeen clones performed significantly better in terms of blackleg resistance than the resistant potato cultivar Glada. Eleven of the selected diploids produced unreduced gametes, which allows their use in improving the resistance of tetraploid potatoes to diseases caused by bacteria of both Pectobacterium and Dickeya.



中文翻译:

二倍体马铃薯种质对茄ic的抗性

马铃薯种植者因不同种类的果胶分解细菌引起的马铃薯软腐和黑腿病而遭受损失。这些细菌在耕种和储藏期间造成产量损失,并可以通过块茎传播给下一代。普遍存在的黑腐atrosepticum赞成有所下降,在过去20年来在欧洲的Dickeya菌P. carotovorum亚种。巴西利亚。筛选了26种二倍体种间茄属杂种,这些杂种先前选择用于块茎抗腐烂马铃薯,对高侵害性梭菌有抗性块茎和黑腿反应方面的应变。用于接种的细菌菌株的特征在于体外具有相对较高的最佳生长温度,范围为33至35°C。与克隆USA 249相比,二十四个二倍体克隆在接种该细菌菌株的块茎反应方面没有差异,USA 249是短杆菌S. brevidens)(+)S. tuberosum的体细胞杂种,已证明对芽孢杆菌具有抗性。就黑腿病抗性而言,十七个克隆的表现明显优于抗性马铃薯品种格拉达。选定的二倍体中有11个产生的配子未减少,因此可用于提高四倍体马铃薯对由两种双歧杆菌细菌引起的疾病的抗性迪卡。

更新日期:2020-10-30
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