Dual oxidase (duox)-derived reactive oxygen species (ROS) have been correlated with neuronal polarity, cerebellar development, and neuroplasticity. However, there have not been many comprehensive studies of the effect of individual duox isoforms on central-axon regeneration in vivo. Here, we explored this question in zebrafish, an excellent model organism for central-axon regeneration studies. In our research, mutation of the duox gene with CRISPR/Cas9 significantly retarded the single-axon regeneration of the zebrafish Mauthner cell in vivo. Using deep transcriptome sequencing, we found that the expression levels of related functional enzymes in mitochondria were down-regulated in duox mutant fish. In vivo imaging showed that duox mutants had significantly disrupted mitochondrial transport and redox state in single Mauthner-cell axon. Our research data provide insights into how duox is involved in central-axon regeneration by changing mitochondrial transport.

双氧化酶 ( duox ) 衍生的活性氧 (ROS) 与神经元极性、小脑发育和神经可塑性相关。然而，关于单个双氧体异构体对体内中心轴突再生的影响的综合研究并不多。在这里，我们在斑马鱼中探索了这个问题，斑马鱼是一种用于中心轴突再生研究的优秀模式生物。在我们的研究中，用 CRISPR/Cas9突变duox基因显着阻碍了体内斑马鱼 Mauthner 细胞的单轴突再生。使用深度转录组测序，我们发现线粒体中相关功能酶的表达水平在duox中下调变异鱼。体内成像表明，duox突变体显着破坏了单个 Mauthner 细胞轴突中的线粒体转运和氧化还原状态。我们的研究数据提供了关于duox如何通过改变线粒体运输参与中枢轴突再生的见解。