Galuteolin, a Chinese herbal medicine, purified from Lonicera Japonica. In this study, we aimed to investigate the neuroprotective effect of galuteolin against cerebral ischemia/reperfusion (I/R) injury. We administered galuteolin or galuteolin and rapamycin to rats which had middle cerebral artery occlusion/reperfusion (MCAO/R). A series of characterizations were carried out to monitor the outcomes of galuteolin in I/R rats regarding the infarct volumes, neurological deficits, and brain water, as well as its effect on neuroprotection and autophagy. It was found that galuteolin significantly reduced the infarct volume, brain water content, and the neurological deficits in a dose-dependent manner. Neuron damages were decreased in the hippocampal carotid artery 1 pyramidal layer by galuteolin. The expression levels of neuron-specific enolase (NSE) increased after galuteolin treatment. Galuteolin significantly decreased the expression levels of autophagy-related proteins. In addition, galuteolin decreased rapamycin-related neuron damages and activations of autophagy in I/R rats. Our data suggested that galuteolin can inhibit ischemic brain injuries through the regulation of autophagy-related indicators in I/R.

Galuteolin，一种从忍冬中提纯的中草药。在这项研究中，我们旨在研究 galuteolin 对脑缺血/再灌注 (I/R) 损伤的神经保护作用。我们对大脑中动脉闭塞/再灌注 (MCAO/R) 的大鼠给予 galuteolin 或 galuteolin 和雷帕霉素。进行了一系列表征以监测 I/R 大鼠中 galuteolin 在梗塞体积、神经功能缺损和脑水方面的结果，以及它对神经保护和自噬的影响。发现 galuteolin 以剂量依赖性方式显着减少梗塞体积、脑含水量和神经功能缺损。galuteolin 减少了海马颈动脉第 1 锥体层的神经元损伤。galuteolin 治疗后神经元特异性烯醇化酶 (NSE) 的表达水平增加。Galuteolin 显着降低自噬相关蛋白的表达水平。此外，galuteolin 减少了 I/R 大鼠中雷帕霉素相关的神经元损伤和自噬的激活。我们的数据表明，galuteolin 可以通过调节 I/R 中的自噬相关指标来抑制缺血性脑损伤。