Changes in land use patterns can lead to changes in soil ecosystem function, and soil microbes could sensitively reflect soil quality and the evolution of different ecosystem functions. In order to study the impact of different land use patterns on microbial community structure, four typical land use patterns, including wetland (WL), meadow (MD), paddy field (Oryza sativa (OS)), and farmland (Zea mays(ZM)), were taken as research objects in Sanjiang Plain. The high-throughput sequencing technology based on bacterial 16S rRNA gene was used to study the community structure of soil bacteria and explore its relationship with soil environmental factors. For alpha diversity, it was found that soil bacterial Simpson index, Chao index, ACE index, and Shannon index in WL were the highest and lowest in OS, while no significant differences of soil bacterial diversity indices were observed among WL, ZM, and MD. The dominant bacterial groups under different land use types were Proteobacteria, Acidobacteria, Chloroflexi, Actinobacteria, and Actinobacteria, and soil bacterial community clearly differed among WL, MD, OS, and ZM. Soil water content, pH, soil organic carbon, total nitrogen, available nitrogen, total phosphorus, available phosphorus, NO3−-N, and soil moisture content had strong effects on soil bacterial community composition. In this perspective, it is well established that different land use patterns could affect microbial communities by changing soil environmental factors.

中文翻译：

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土地利用方式对三江平原湿地土壤细菌群落结构及多样性的影响

土地利用方式的变化会导致土壤生态系统功能的变化，土壤微生物可以敏感地反映土壤质量和不同生态系统功能的演变。为研究不同土地利用方式对微生物群落结构的影响，选取湿地(WL)、草甸(MD)、稻田(Oryza sativa (OS))、农田(Zea mays(ZM))四种典型土地利用方式))，作为三江平原的研究对象。采用基于细菌16S rRNA基因的高通量测序技术研究土壤细菌群落结构，探索其与土壤环境因子的关系。对于α多样性，发现WL中土壤细菌Simpson指数、Chao指数、ACE指数和Shannon指数最高，OS最低，而 WL、ZM 和 MD 之间的土壤细菌多样性指数没有显着差异。不同土地利用类型下的优势菌群为变形菌属、酸菌属、绿屈菌属、放线菌属和放线菌属，WL、MD、OS 和 ZM 之间的土壤细菌群落存在明显差异。土壤含水量、pH、土壤有机碳、全氮、速效氮、全磷、速效磷、NO3--N和土壤水分含量对土壤细菌群落组成有很强的影响。从这个角度来看，众所周知，不同的土地利用模式可以通过改变土壤环境因素来影响微生物群落。放线菌、放线菌和土壤细菌群落在 WL、MD、OS 和 ZM 之间明显不同。土壤含水量、pH、土壤有机碳、全氮、速效氮、全磷、速效磷、NO3--N和土壤水分含量对土壤细菌群落组成有很强的影响。从这个角度来看，众所周知，不同的土地利用模式可以通过改变土壤环境因素来影响微生物群落。放线菌、放线菌和土壤细菌群落在 WL、MD、OS 和 ZM 之间明显不同。土壤含水量、pH、土壤有机碳、全氮、速效氮、全磷、速效磷、NO3--N和土壤水分含量对土壤细菌群落组成有很强的影响。从这个角度来看，众所周知，不同的土地利用模式可以通过改变土壤环境因素来影响微生物群落。