Aphanizomenon flos-aquae, a freshwater cyanobacterium harvested from Upper Klamath Lake, Oregon, USA, is employed as a functional food material. Aphanizomenon flos-aquae can be fermented with Lactobacillus plantarum and Lactococcus lactis subsp. lactis. Here, to determine the dietary effects of A. flos-aquae (AFA) and fermented A. flos-aquae (F-AFA) on the mouse gut microbiome, high-sucrose diet containing no dietary fibre (NF), 5% AFA or 5% F-AFA was administered to ICR mice for 14 days. Compared with those in mice fed the NF, lower body weight gains and epididymal fat pad tissue weights were observed in mice fed the diet containing F-AFA. After feeding, the caecal microbiome was analysed by 16S rRNA (V4) gene amplicon sequencing using the Illumina MiSeq system. Sequences were clustered into operational taxonomic units, with a 97% identity cutoff, using the QIIME2 workflow script and SILVA database. The caecal microbiome alpha diversity was high in mice fed the F-AFA diet. The abundances of Muribaculum- and Alistipes inops-like bacteria belonging to the phylum Bacteroidetes and Ruminococcaceae UCG-014-like bacteria belonging to phylum Firmicutes were higher in the F-AFA group than in the AFA group. Isolation of typical F-AFA-susceptible gut indigenous bacteria and functional studies of the isolates in the presence of F-AFA are warranted to validate the current findings.

从美国俄勒冈州上克拉马斯湖收获的淡水蓝细菌Aphanizomenon flos-aquae被用作功能性食品原料。可以用植物乳杆菌和乳酸乳球菌亚种发酵大花露水酵母。乳酸菌。在这里，要确定A. flos-aquae（AFA）和发酵的A. flos-aquae的饮食效果在小鼠肠道微生物组中添加（F-AFA），将不含饮食纤维（NF），5％AFA或5％F-AFA的高蔗糖饮食施用给ICR小鼠14天。与饲喂NF的小鼠相比，饲喂含F-AFA的小鼠的体重增加和附睾脂肪垫组织重量较低。喂食后，使用Illumina MiSeq系统通过16S rRNA（V4）基因扩增子测序分析盲肠微生物组。使用QIIME2工作流程脚本和SILVA数据库，将序列聚类为可操作的分类单位，同一性截止值为97％。饲喂F-AFA饮食的小鼠的盲肠微生物组α多样性很高。的丰度Muribaculum -和Alistipes inops样的细菌属于拟杆菌门和RuminococcaceaeF-AFA组中属于硬毛菌门的类UCG-014细菌要高于AFA组。分离典型的F-AFA易感肠道土著细菌，并在F-AFA存在下对分离株进行功能研究，以验证当前的发现。