Staphylococcus aureus is a most important reason of bacterial keratitis. The emergence of S. aureus resistance to methicillin necessitates searching for new antimicrobial components for keratitis. CM11 is a cationic peptide with strong antibacterial activity against a range of bacteria. In this study, in vitro bactericidal activity of CM11 was investigated using the time-killing assay. For this purpose, corneal methicillin-resistant S. aureus (MRSA)-infected rabbit models were experimentally developed through intrastromal injection of bacteria. The infected rabbits were treated in three groups by artificial tear, gentamicin, and CM11, and their conjunctiva, iris, and cornea were clinically examined using a slit lamp and histopathological examination. The variance analyses of microbial (colony counts) and pathological examinations on the harvested cornea samples showed a significant improvement in the treated groups (CM11 and gentamicin) compared to the control eyes (P ≤ 0.05). According to findings, CM11 and gentamicin could significantly reduce the CFU in comparison with the group received artificial tear. The mean bacterial counts (log CFU/ml) from corneal culture were 2.1, 5.02, and 8.89 for gentamicin, CM11, and control group, respectively (P ≤ 0.05). The above-mentioned findings displayed the efficacy of CM11 cationic peptide for curing the MRSA-mediated keratitis.

金黄色葡萄球菌是细菌性角膜炎的最重要原因。金黄色葡萄球菌对甲氧西林的抗药性的出现使得必须寻找新的角膜炎抗菌成分。CM11是一种阳离子肽，对多种细菌具有很强的抗菌活性。在这项研究中，使用时间杀灭试验研究了CM11的体外杀菌活性。为此，耐角膜甲氧西林的金黄色葡萄球菌（MRSA）感染的兔模型是通过基质内注射细菌实验开发的。将感染的兔子分为三组，分别用人工泪液，庆大霉素和CM11进行治疗，并使用裂隙灯和组织病理学检查对它们的结膜，虹膜和角膜进行临床检查。微生物的方差分析（菌落计数），并且在收获的角膜样品病理学检查显示相较于对照眼（在处理过的基团的显著改善（CM11和庆大霉素）P  ≤0.05）。根据发现，与接受人工泪液治疗的组相比，CM11和庆大霉素可以显着降低CFU。对于庆大霉素，CM11和对照组，来自角膜培养的平均细菌数（log CFU / ml）分别为2.1、5.02和8.89（P  ≤0.05）。上述发现显示了CM11阳离子肽治疗MRSA介导的角膜炎的功效。