Pufferfish belonging to Lagocephalus are composed of several species, some of which have been recognized to be non-toxic. Although fish belonging to this genus inhabit a wide variety of seawaters from temperate to tropical, toxin distribution has remained unclear. The present study was conducted to thoroughly survey the presence of tetrodotoxin (TTX) and its analogs (TTXs) in the extracts from various tissues of Lagocephalus pufferfish, including L. spadiceus, L. cheesemanii, L. lunaris, and L. inermis collected in Vietnam, by using the TTX enzyme-linked immunosorbent assay (ELISA) kit. Analyses using the TTX-ELISA kit demonstrated the presence of a considerable amount of TTXs in the extracts prepared from various tissues and organs of Lagocephalus pufferfish. TTX was detected in gonads and intestine of L. cheesemanii as well as L. lunaris by high performance liquid chromatography with fluorescence detection, but not in any tissues and organs of L. spadiceus. The extract from muscle of L. spadiceus was further subjected to liquid chromatography-mass spectrometry analyses to investigate toxin components, revealing the presence of a high amount of 5,6,11-trideoxyTTX (TDT), but no TTX. Since the toxicity of TDT is very weak, L. spadiceus muscle was regarded to be non-toxic as far as samples collected in the present study are concerned.

中文翻译：

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河豚毒素及其类似物在越南采集的河豚鱼中的组织分布

属于 Lagocephalus 的河豚由几种物种组成，其中一些已被认​​为是无毒的。尽管属于该属的鱼类栖息在从温带到热带的各种海水中，但毒素分布仍不清楚。本研究旨在彻底调查河豚毒素 (TTX) 及其类似物 (TTX) 在来自河豚不同组织的提取物中的存在，包括 L. spadiceus、L. cheesemanii、L. lunaris 和 L. inermis。越南，通过使用 TTX 酶联免疫吸附测定 (ELISA) 试剂盒。使用 TTX-ELISA 试剂盒进行的分析表明，从河豚各种组织和器官制备的提取物中存在大量 TTX。在 L. cheesemanii 和 L. 的性腺和肠道中检测到 TTX。lunaris 通过具有荧光检测的高效液相色谱法检测到，但在 L. spadiceus 的任何组织和器官中均未检测到。L. spadiceus 肌肉提取物进一步进行液相色谱-质谱分析以研究毒素成分，揭示存在大量 5,6,11-trideoxyTTX (TDT)，但没有 TTX。由于 TDT 的毒性非常弱，因此就本研究收集的样本而言，L. spadiceus 肌肉被认为是无毒的。