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Draft genome sequence of Promicromonospora panici sp. nov., a novel ionizing-radiation-resistant actinobacterium isolated from roots of the desert plant Panicum turgidum
Extremophiles ( IF 2.6 ) Pub Date : 2020-10-26 , DOI: 10.1007/s00792-020-01207-8
Sihem Guesmi , Imen Nouioui , Petar Pujic , Audrey Dubost , Afef Najjari , Kais Ghedira , José M. Igual , Ameur Cherif , Hans-peter Klenk , Haïtham Sghaier , Philippe Normand

A novel strain of the genus Promicromonospora, designated PT9T, was recovered from irradiated roots of the xerophyte Panicum turgidum collected from the Ksar Ghilane oasis in southern Tunisia. Strain PT9T is aerobic, non-spore-forming, Gram- positive actinomycete that produces branched hyphae and forms white to yellowish-white colonies. Chemotaxonomic features, including fatty acids, whole cell sugars and polar lipid profiles, support the assignment of PT9T to the genus Promicromonospora. The genomic relatedness indexes based on DNA-DNA hybridization and average nucleotide identity values revealed a significant genomic divergence between strain PT9T and all sequenced type strains of the taxon. Phylogenomic analysis showed that isolate PT9T was most closely related to Promicromonospora soli CGMCC 4.7398T. Phenotypic and phylogenomic analyses suggest that isolate PT9T represents a novel species of the genus Promicromonospora, for which the name Promicromonospora panici sp. nov. is proposed. The type strain is PT9T (LMG 31103T = DSM 108613T).The isolate PT9T is an ionizing-radiation-resistant actinobacterium (D10 value = 2.6 kGy), with resistance to desiccation and hydrogen peroxide. The complete genome sequence of PT9T consists of 6,582,650 bps with 71.2% G+C content and 6291 protein-coding sequences. This genome will help to decipher the microbial genetic bases for ionizing-radiation resistance mechanisms including the response to oxidative stress.



中文翻译:

草拟小单孢菌的基因组序列草案。nov。,从沙漠植物Panicum turgidum的根中分离出的新型抗电离辐射放线菌

属的新菌株 原小,指定PT9 Ť,从旱生的照射根回收柳枝圆锥小麦从突尼斯南部的Ksar Ghilane绿洲收集。PT9 T菌株是需氧的,无芽孢的革兰氏阳性放线菌,产生分支的菌丝并形成白色至黄白色菌落。化学分类学特征,包括脂肪酸,全细胞糖和极性脂质谱,支持PT9 T属于Promicromonospora属。基于DNA-DNA杂交和平均核苷酸同一性值的基因组相关性指数显示菌株PT9 T之间存在显着的基因组差异以及所有分类单元的分类株。Phylogenomic分析表明,分离PT9牛逼是最密切相关的原小索利CGMCC 4.7398牛逼。表型和系统基因组学分析表明,分离的PT9 T代表了Promicromonospora属的一种新物种,其名称为 Promicromonospora panici sp。十一月 被提议。类型菌株为PT9 T(LMG 31103 T  = DSM 108613 T).PT9 T分离株是抗电离辐射的放线菌(D 10值= 2.6 kGy),具有抗干燥性和过氧化氢性能。PT9 T的完整基因组序列由6,582,650 bps和71.2%的G + C含量以及6291个蛋白质编码序列组成。该基因组将有助于破译微生物的遗传基础,用于抗电离辐射的机制,包括对氧化应激的反应。

更新日期:2020-10-30
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