Sewage effluent effects on the biochemical parameters of Astyanax bimaculatus organs were investigateted. Treated sewage was collected in a treatment plant; 43 compounds, among them, pharmaceuticals and hormones, were investigated. Caffeine, ciprofloxacin, clindamycin, ofloxacin, oxytetracycline, paracetamol, sulfadiazine, sulfamethoxazole, sulfathiazole and tylosin waste was detected in the collected material. Fish were divided into four groups: control, TSE (treated sewage effluent), TSE + P (TSE with increased concentration of five pharmaceuticals) and PTSE (TSE + P post-treated with O₃/H₂O₂/UV). Biochemical parameters were evaluated in different organs after 14-day exposure. TBARS levels increased significantly in the brain of animals in the TSE and TSE + P groups in comparison to the control. There was significant reduction in TBARS levels recorded for the liver, muscle and gills of animals in the PTSE group in comparison to those of animals in the other groups. AChE activity reduced in the muscle of animals in the groups showing the highest pharmaceutical concentrations. CAT activity in the liver of animals in groups exposed to pharmaceutical effluent was inhibited. GST activity increased in brain of animals in the TSE + P and PTSE groups, whereas reduced levels of this activity were observed in liver of animals in the TSE group. Increased GST activity was observed in the brain of animals in TSE + P and PTSE groups. Based on integrated biomarker response values, the TSE + P group presented greater changes in the analyzed parameters. Results point out that pharmaceutical waste can cause oxidative stress, as well as affect biochemical and enzymatic parameters in Astyanax sp. Post-treatment can also reduce damages caused to fish, even in case of the likely formation of metabolites. Based on these results, these metabolites can be less toxic than the original compounds; however, they were not able to fully degrade the pharmaceutical waste found in the sewage, which can interfere in fish metabolism.

研究了污水对双足连翘器官生化指标的影响。处理后的污水收集在处理厂中；研究了43种化合物，其中包括药物和激素。在收集的材料中检测到咖啡因，环丙沙星，克林霉素，氧氟沙星，土霉素，对乙酰氨基酚，磺胺嘧啶，磺胺甲恶唑，磺胺噻唑和泰乐菌素废物。鱼分为四组：对照组，TSE（处理后的污水），TSE + P（增加了五种药物浓度的TSE）和PTSE（TSE + P用O ₃ / H ₂ O _{2后处理）}/ UV）。暴露14天后，在不同器官中评估生化参数。与对照组相比，TSE和TSE + P组的动物大脑中的TBARS水平显着增加。与其他组的动物相比，PTSE组的动物的肝脏，肌肉和g的TBARS水平显着降低。在显示最高药物浓度的组中，动物肌肉中的AChE活性降低。暴露于药物流出物中的动物肝脏中的CAT活性受到抑制。在TSE + P和PTSE组中，动物脑中的GST活性增加，而在TSE组中，动物肝脏中的GST活性降低。在TSE + P和PTSE组的动物大脑中观察到GST活性增加。基于综合的生物标志物反应值，TSE + P组在分析参数方面表现出更大的变化。结果指出，制药废料可以引起氧化应激，并影响大豆的生化和酶参数。Astyanax sp。即使可能形成代谢物，后处理也可以减少对鱼类的损害。根据这些结果，这些代谢物的毒性可能小于原始化合物。但是，它们不能完全降解污水中发现的制药废料，这些废料可能会干扰鱼类的新陈代谢。