Abstract

In the present study, we assessed the role of annexin 13 membrane-binding protein (ANXA13) in the intracellular transport of vesicles containing type II ribosome-inactivating proteins (RIP-IIs). A modified human intestinal epithelial cell line HT29 was used, in which the expression of ANXA13 was significantly reduced. The cytotoxic effect of ricin and viscumin was evaluated by modification of 28S ribosome RNA. The observed differences in the activity of toxins on the parental and modified HT29 lines indicate that ANXA13 plays a different role in the intracellular transport of vesicles containing the RIP-IIs.

中文翻译：

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核糖体失活蛋白的细胞内运输取决于膜联蛋白13。

摘要

在本研究中，我们评估了膜联蛋白13膜结合蛋白（ANXA13）在包含II型核糖体失活蛋白（RIP-IIs）的囊泡的细胞内运输中的作用。使用修饰的人肠上皮细胞系HT29，其中ANXA13的表达显着降低。通过修饰28S核糖体RNA评估蓖麻毒蛋白和粘蛋白的细胞毒性作用。在亲本和修饰的HT29品系上观察到的毒素活性差异表明，ANXA13在含有RIP-II的囊泡的细胞内运输中起着不同的作用。