Abstract

Proliferation of mammalian cells is often accompanied by an increase in the content of the nucleolar proteins, which allows researchers to consider such proteins as potential activation markers. To test this assumption experimentally, we examined the expression pattern of the nucleolar rRNA processing factor SURF6 in normal (resting) peripheral blood lymphocytes, lymphocytes activated for proliferation in vitro, and in blood samples from patients with lymphoproliferative diseases. Using two methods (immunofluorescence and immunoblotting), we for the first time showed that the SURF6 protein is not detected in normal lymphocytes but can easily be visualized in lymphocytes after PHA activation and in lymphocytes of lymphocytic leukemia patients. The level of SURF6 expression in patients correlated with the aggressiveness of the disease development determined by the content of Ki-67-positive lymphocytes. These results allow the SURF6 nucleolar protein to be considered as a putative activation marker of lymphocytes in human blood disorders.

中文翻译：

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人正常活化淋巴细胞和淋巴细胞增生性疾病患者淋巴细胞中核仁蛋白SURF6的表达水平较高

摘要

哺乳动物细胞的增殖通常伴随着核仁蛋白含量的增加，这使研究人员可以将此类蛋白视为潜在的激活标记。为了通过实验验证这一假设，我们检查了核仁rRNA加工因子SURF6在正常（静止）外周血淋巴细胞，体外活化增殖的淋巴细胞以及患有淋巴细胞增生性疾病的患者血液样本中的表达模式。我们首次使用两种方法（免疫荧光法和免疫印迹法）显示，在正常淋巴细胞中未检测到SURF6蛋白，但在PHA活化后的淋巴细胞和淋巴细胞性白血病患者的淋巴细胞中很容易观察到SURF6蛋白。患者中SURF6表达的水平与疾病发展的侵袭性有关，后者由Ki-67阳性淋巴细胞的含量决定。这些结果使得SURF6核仁蛋白被认为是人类血液疾病中淋巴细胞的假定激活标记。