Bacterial cellulose has unique structural, functional, physical and chemical properties. The mass production of bacterial cellulose for industrial application has recently become more popular and attractive. The aim of this study was to develop an efficient scale-up process of bacterial cellulose production. Bacterial cellulose productivity of a new isolate Acetobacter pasteurianus RSV-4 (MTCC 25117) was investigated in whey medium without addition of any additives. Produced bacterial cellulose was characterized for its structure, purity, thermostability and strength by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermogravimetric analysis and Differential scanning calorimetry. β-galactosidase (1.5 IU/ml) was used to split whey lactose into its corresponding monomers glucose and galactose. Glucose was specifically consumed by Acetobacter pasteurianus for the production of BC. The highest concentration of 5.6 g cellulose/L of whey was obtained at 30 °C after 8 days of bacterial growth. Process was optimized for the production of bacterial cellulose on 120 L of whey medium under static conditions. Whey, a waste byproduct of milk processing industry was used in the process and therefore the developed process might be economical.

细菌纤维素具有独特的结构，功能，物理和化学特性。用于工业应用的细菌纤维素的大规模生产近来变得越来越流行和有吸引力。这项研究的目的是开发一种有效的细菌纤维素生产规模放大方法。新型分离巴斯德醋杆菌的细菌纤维素生产力在不添加任何添加剂的乳清培养基中研究了RSV-4（MTCC 25117）。通过傅立叶变换红外光谱，X射线衍射，扫描电子显微镜，热重分析和差示扫描量热法对制得的细菌纤维素的结构，纯度，热稳定性和强度进行了表征。β-半乳糖苷酶（1.5 IU / ml）用于将乳清乳糖分裂成其相应的单体葡萄糖和半乳糖。葡萄糖被巴氏醋杆菌专门消耗用于生产BC。细菌生长8天后，在30°C时，乳清的最高浓度为5.6 g纤维素/ L。优化了在静态条件下在120 L乳清培养基上生产细菌纤维素的工艺。该过程中使用了乳清，这是牛奶加工业的废副产品，因此开发的过程可能是经济的。