Nanofibrillated cellulose (NFC) extracted from biomass has potential applications in material science and biomedical engineering. In this study, NFC was obtained from bleached eucalyptus Kraft pulp (BEKP) using two commercial enzyme cocktails with cellulolytic and hemicellulolytic activities and non-catalytic protein (swollenin), followed by ultrasonication. This work represents an initial study of the implementation of non-catalytic proteins along with enzymes to extract NFC from biomass. Enzymatic pretreatment was performed to partially remove hemicellulose while enhancing cellulose accessibility for NFC extraction. Cellulase pretreatment with xylanase and swollenin supplementation increased cellulose accessibility and fiber swelling due to extensive hemicellulose removal (> 80%) and fiber morphology changes. Subsequent ultrasonication was performed for cellulose nanofibrillation resulting in high NFC yields (61–97%), while keeping NFC properties almost unchanged. Through this process, cellulose nanofibers with diameters ranging from 3 nm to 10 nm were effectively isolated from BEKP, which allows to produce high quality NFC for further applications.

从生物质中提取的纳米原纤化纤维素（NFC）在材料科学和生物医学工程中具有潜在的应用。在这项研究中，使用两种具有纤维素分解和半纤维素分解活性以及非催化蛋白（膨胀素）的商业化酶混合物，从漂白的桉木牛皮纸浆（BEKP）中获得NFC，然后进行超声波处理。这项工作代表了对非催化蛋白以及酶从生物质中提取NFC的实施的初步研究。进行了酶预处理以部分除去半纤维素，同时增强了NFC提取中纤维素的可及性。由于广泛去除半纤维素（> 80％）和纤维形态变化，用木聚糖酶和膨胀素补充的纤维素酶预处理增加了纤维素的可及性和纤维溶胀。随后对纤维素纳米纤丝进行超声波处理，从而获得较高的NFC收率（61％至97％），同时保持NFC特性几乎不变。通过该过程，有效地从BEKP中分离出了直径范围为3 nm至10 nm的纤维素纳米纤维，从而可以生产出高质量的NFC以便进一步应用。