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First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766)
Brazilian Journal of Microbiology ( IF 2.1 ) Pub Date : 2020-10-23 , DOI: 10.1007/s42770-020-00389-9
Caroline Giuseppa Spera 1 , Fernanda Louise Pereira Lavorente 1 , Elis Lorenzetti 1, 2, 3 , Gustavo de Calasans Marques 4 , Luana de Almeida Freitas 1 , Maíra Bonamin Martins 5 , Carlos Roberto Teixeira 4 , Amauri Alcindo Alfieri 1, 2 , Alice Fernandes Alfieri 1, 2
Affiliation  

The crab-eating fox (Cerdocyon thous) is a small wild mammal present in all Brazilian biomes and in some countries of South America. This study aimed to verify the involvement of viral infectious agents in the death of a wild crab-eating fox pup (Cerdocyon thous) in Brazil. The Center for Medicine and Research of Wild Animals of the Universidade Estadual Paulista received a free-living crab-eating fox aged approximately 21 days and apparently healthy. After 13 days, the animal presented anorexia, diarrhea, fever, prostration, and neurological signs progressing to death with an inconclusive diagnosis. In a retrospective study, tissue fragments stored at - 80 °C were used to identify nucleic acids from major canine viruses, such as canine parvovirus-2 (CPV-2), canine adenovirus A types 1 and 2, canid alphaherpesvirus 1, and canine distemper virus. The amplified product with the expected length for CPV-2 was obtained from the heart fragment. After performing nucleotide (nt) sequencing of the amplicon, it was possible to demonstrate that the crab-eating fox strain exhibited high (99.8%) nt identity with the CPV-2b prototype (CPV-39 strain). Additionally, deduced amino acid (aa) sequence analysis showed the GAT codon for the aa Asp (D) at position 426 of the CPV-2 viral protein VP2, which characterizes the subtype 2b. To the best of the authors' knowledge, this report describes the first detection of CPV-2b DNA in tissue fragments from a crab-eating fox.

中文翻译:

在食蟹狐狸幼崽中首次检测到犬细小病毒 2b DNA (Cerdocyon thous, Linnaeus, 1766)

食蟹狐 (Cerdocyon tous) 是一种小型野生哺乳动物,存在于所有巴西生物群落和南美洲的一些国家。这项研究旨在验证病毒感染因子是否参与了巴西一只吃螃蟹的野生狐狸幼崽 (Cerdocyon thou) 的死亡。埃斯塔杜尔保利斯塔大学野生动物医学和研究中心收到了一只自由生活的食蟹狐狸,大约 21 天,看起来很健康。13 天后,该动物出现厌食、腹泻、发烧、虚脱和神经系统体征,最终诊断未定。在一项回顾性研究中,储存在 - 80 °C 下的组织碎片用于鉴定来自主要犬病毒的核酸,例如犬细小病毒 2 (CPV-2)、犬腺病毒 A 1 型和 2 型、犬甲疱疹病毒 1 和犬瘟热病毒。从心脏片段获得具有CPV-2预期长度的扩增产物。在对扩增子进行核苷酸 (nt) 测序后,可以证明食蟹狐狸菌株与 CPV-2b 原型 (CPV-39 菌株) 表现出高 (99.8%) nt 同一性。此外,推导的氨基酸 (aa) 序列分析显示 CPV-2 病毒蛋白 VP2 的第 426 位上的 aa Asp (D) 的 GAT 密码子是 2b 亚型的特征。据作者所知,本报告描述了首次在食蟹狐狸的组织碎片中检测到 CPV-2b DNA。8%) nt 与 CPV-2b 原型 (CPV-39 菌株) 相同。此外,推导的氨基酸 (aa) 序列分析显示 CPV-2 病毒蛋白 VP2 的第 426 位上的 aa Asp (D) 的 GAT 密码子是 2b 亚型的特征。据作者所知,本报告描述了首次在食蟹狐狸的组织碎片中检测到 CPV-2b DNA。8%) nt 与 CPV-2b 原型 (CPV-39 菌株) 相同。此外,推导的氨基酸 (aa) 序列分析显示 CPV-2 病毒蛋白 VP2 的第 426 位上的 aa Asp (D) 的 GAT 密码子是 2b 亚型的特征。据作者所知,本报告描述了首次在食蟹狐狸的组织碎片中检测到 CPV-2b DNA。
更新日期:2020-10-23
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