OBJECTIVE Fructooligosaccharides (FOS) are prebiotic substances that have been extensively incorporated in different products of food industry mostly for their bifidogenic properties and economic value. The main commercial FOS production comes from the biotransformation of sucrose and intracellular and extracellular microbial enzymes-fructosyltransferases (FTase). Aspergillus oryzae IPT-301 produces FTase. In order to increase its production, this study focuses on evaluating the effects of different agitation speed and aeration rates which affect yields in a stirred tank bioreactor. RESULTS Agitation had more influence on cell growth than aeration. The maximum intracellular FTase activity and the volumetric productivity of total intracellular FTase were obtained at 800 rpm and 0.75 vvm, and reached values of 2100 U g-1 and 667 U dm-3 h-1, respectively. The agitation speed had a strong influence on the activity of extracellular FTase produced which reached the maximum amount of 53 U cm-3. The higher value of total activity obtained was 22,831 U dm-3 at 0.75 vvm and 800 rpm. CONCLUSION Aeration rates and agitation speed showed strong influence upon the growth and production of fructosyltransferase from Aspergillus oryzae IPT-301 in media containing sucrose as carbon source. The control of aeration rate and agitation speed can be a valuable fermentation strategy to improve enzyme production.

中文翻译：

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搅拌速度和曝气速率对米曲霉IPT-301在搅拌釜生物反应器中果糖基转移酶产量的影响

目的 低聚果糖 (FOS) 是益生元物质，已广泛应用于食品工业的不同产品中，主要是因为它们的双歧特性和经济价值。主要的商业 FOS 生产来自蔗糖和细胞内和细胞外微生物酶 - 果糖基转移酶 (FTase) 的生物转化。米曲霉 IPT-301 产生 FTase。为了提高产量，本研究侧重于评估不同搅拌速度和曝气速率对搅拌罐生物反应器产量的影响。结果 搅拌比通气对细胞生长的影响更大。在 800 rpm 和 0.75 vvm 下获得最大细胞内 FTase 活性和总细胞内 FTase 的容积生产率，并达到 2100 U g-1 和 667 U dm-3 h-1 的值，分别。搅拌速度对产生的胞外FTase活性有很大影响，达到最大值53 U cm-3。在 0.75 vvm 和 800 rpm 下获得的总活性的较高值是 22,831 U dm-3。结论曝气速率和搅拌速度对米曲霉IPT-301在含蔗糖作为碳源的培养基中的果糖基转移酶的生长和产生有很大影响。控制曝气速率和搅拌速度是提高酶产量的重要发酵策略。结论曝气速率和搅拌速度对米曲霉IPT-301在含蔗糖作为碳源的培养基中的果糖基转移酶的生长和产生有很大影响。控制曝气速率和搅拌速度是提高酶产量的重要发酵策略。结论曝气速率和搅拌速度对米曲霉IPT-301在含蔗糖作为碳源的培养基中的果糖基转移酶的生长和产生有很大影响。控制曝气速率和搅拌速度是提高酶产量的重要发酵策略。