Abstract The aim of this work was to construct a new type of system for the green extraction and efficient separation of Ginkgo biloba leaf polyprenols (GBP). First, cell disruption technologies (enzymolysis, ultrasound, microwave and ball milling) coupled with deep eutectic solvents (DES) were used to extract polyprenyl acetates (PA) from Ginkgo biloba leaves. Results showed that ball milling was the best assisted extraction method, and the amount of PA was 89.88 mg/g. Then, a novel silver-loaded material with selectivity for PA was synthesized, characterized and utilized to absorb PA from the DES extraction solution. The purity of the PA quickly reached 71.28% after being separated by the silver-loaded material. Finally, saponification and molecular distillation were adopted to remove non-GBP ingredients, and a high-purity GBP (99.80%) was obtained by optimizing its processing conditions. This study provides a technical support for the development of a green and efficient GBP separation method and lays the foundation for the fabrication of GBP-based drugs, health products and cosmetics.

中文翻译：

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银杏叶聚异戊二烯醇分离绿色高效体系的构建

摘要 本工作的目的是构建一种新型的银杏叶聚戊烯醇（GBP）绿色提取和高效分离系统。首先，细胞破碎技术（酶解、超声、微波和球磨）与低共熔溶剂 (DES) 相结合，用于从银杏叶中提取聚醋酸乙烯酯 (PA)。结果表明，球磨是最好的辅助提取方法，PA的量为89.88 mg/g。然后，合成了一种对 PA 具有选择性的新型载银材料，表征并用于从 DES 提取溶液中吸收 PA。PA的纯度在被载银材料分离后迅速达到71.28%。最后通过皂化和分子蒸馏去除非GBP成分，得到高纯度GBP（99. 80%)是通过优化其加工条件获得的。该研究为开发绿色高效的GBP分离方法提供了技术支持，为GBP基药物、保健品和化妆品的制备奠定了基础。