Background

Stress may be one of the main causes of fear and anxiety. Previous studies have shown that the nucleus accumbens is involved in emotional responses. However, in the nucleus accumbens, the mRNA and miRNA profiles of stress susceptibility and resilience of psychological stress still need to be studied.

Materials and methods

In this study, by observing the conspecific being attacked, the witness group experienced psychological stress. After five days of psychological stress, the fear memory of mice was measured by social interaction test, and the degree of anxiety was measured by elevated plus maze. mRNA and miRNA profiles in the nucleus accumbens tissue of control, susceptible and resilient mice were established by high-throughput sequencing.

Results

In susceptible mice versus resilient mice, the Differentially expressed genes (DEGs) may be related to psychological stress-induced susceptibility. DEGs enriched in Cell adhesion molecules, Neuroactive ligand-receptor interaction, Gap junction, PI3K-Akt, VEGF, Jak-STAT, Ras, and Chemokine pathways were up-regulated. DEGs enriched in cGMP-PKG, B cell receptor, and NOD-like receptor pathways were down- regulated. The sequencing results of mRNAs and miRNAs were verified by qRT-PCR and dual luciferase reporter assay.

Conclusion

The imbalance of different synapses and pathways in the nucleus accumbens may be related to susceptibility and resilience caused by psychological stress.

中文翻译：

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伏伏核中的mRNA和miRNA谱与心理应激诱导的易感和有弹性的小鼠有关

背景

压力可能是恐惧和焦虑的主要原因之一。先前的研究表明伏隔核参与情绪反应。然而，在伏伏核中，应激易感性和心理应激适应力的mRNA和miRNA图谱仍然需要研究。

材料和方法

在这项研究中，通过观察被攻击者的具体情况，证人群体遭受了心理压力。经过五天的心理压力后，通过社交互动测试测量小鼠的恐惧记忆，并通过高架迷宫测量焦虑程度。通过高通量测序建立了对照，易感和有弹性的小鼠伏隔核组织中的mRNA和miRNA图谱。

结果

在易感小鼠与有恢复力的小鼠中，差异表达基因（DEG）可能与心理压力诱发的易感性有关。富含细胞粘附分子，神经活性配体-受体相互作用，Gap连接，PI3K-Akt，VEGF，Jak-STAT，Ras和趋化因子途径的DEGs上调。富含cGMP-PKG，B细胞受体和NOD样受体途径的DEGs被下调。通过qRT-PCR和双重荧光素酶报告基因分析验证了mRNA和miRNA的测序结果。

结论

伏隔核中不同突触和途径的不平衡可能与心理压力引起的易感性和适应力有关。