Ambystoma mexicanum (axolotl) has been one of the major experimental models for the study of regeneration during the past 100 years. Axolotl limb regeneration takes place through a multi-stage and complex developmental process called epimorphosis that involves diverse events of cell reprogramming. Such events start with dedifferentiation of somatic cells and the proliferation of quiescent stem cells to generate a population of proliferative cells called blastema. Once the blastema reaches a mature stage, cells undergo progressive differentiation into the diverse cell lineages that will form the new limb. Such pivotal cell reprogramming phenomena depend on the fine-tuned regulation of the cell cycle in each regeneration stage, where cell populations display specific proliferative capacities and differentiation status. The axolotl genome has been fully sequenced and released recently, and diverse RNA-seq approaches have also been generated, enabling the identification and conservatory analysis of core cell cycle regulators in this species. We report here our results from such analyses and present the transcriptional behavior of key regulatory factors during axolotl limb regeneration. We also found conserved protein interactions between axolotl Cyclin Dependent Kinases 2, 4 and 6 and Cyclins type D and E. Canonical CYC-CDK interactions that play major roles in modulating cell cycle progression in eukaryotes.

中文翻译：

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墨西哥 Ambystoma 肢体再生过程中核心细胞周期调节因子及其转录行为的保守性分析

Ambystoma mexiconum (axolotl) 是过去 100 年来研究再生的主要实验模型之一。Axolotl 肢体再生是通过称为表观发育的多阶段和复杂的发育过程进行的，该过程涉及细胞重编程的各种事件。此类事件始于体细胞的去分化和静止干细胞的增殖，以产生称为胚细胞的增殖细胞群。一旦胚泡达到成熟阶段，细胞就会逐渐分化为不同的细胞谱系，这些细胞谱系将形成新的肢体。这种关键的细胞重编程现象取决于每个再生阶段对细胞周期的微调调节，其中细胞群显示出特定的增殖能力和分化状态。蝾螈基因组最近已被完全测序并发布，并且还产生了多种 RNA-seq 方法，从而能够对该物种的核心细胞周期调节因子进行鉴定和保存分析。我们在此报告这些分析的结果，并展示了蝾螈肢体再生过程中关键调节因子的转录行为。我们还发现了蝾螈细胞周期蛋白依赖性激酶 2、4 和 6 与细胞周期蛋白 D 和 E 型之间的保守蛋白质相互作用。典型的 CYC-CDK 相互作用在调节真核生物的细胞周期进程中起主要作用。我们在此报告这些分析的结果，并展示了蝾螈肢体再生过程中关键调节因子的转录行为。我们还发现了蝾螈细胞周期蛋白依赖性激酶 2、4 和 6 与细胞周期蛋白 D 和 E 型之间的保守蛋白质相互作用。典型的 CYC-CDK 相互作用在调节真核生物的细胞周期进程中起主要作用。我们在此报告这些分析的结果，并展示了蝾螈肢体再生过程中关键调节因子的转录行为。我们还发现了蝾螈细胞周期蛋白依赖性激酶 2、4 和 6 与细胞周期蛋白 D 和 E 型之间的保守蛋白质相互作用。典型的 CYC-CDK 相互作用在调节真核生物的细胞周期进程中起主要作用。