Owing to the side effects of current drugs for treating atopic dermatitis (AD), a chronic disease in the skin, traditional herbal medicine is receiving much attention as an alternative treatment. Asarum sieboldii has traditionally been used to treat colds, fevers, headaches, coughs, neuralgia, chronic bronchitis, asthma, and allergies. In this study, 6 compounds (echinacoside, vanillic acid, kakuol, methyl eugenol, sesamin, and asarinin) in A. sieboldii were analyzed simultaneously using high-performance liquid chromatography (HPLC), and the proposed analytical method was validated. In addition, the inhibitory effects of the A. sieboldii extract and the 6 analyzed compounds on the expression of chemokine were evaluated in HaCaT cells. The HPLC method showed high linearity, with a correlation coefficient of ≥0.9999. The limits of detection for the 6 compounds ranged from 0.00 to 0.02 µg/mL, and the limits of quantification ranged from 0.01 to 0.05 µg/mL. The intraday and interday precisions were 0.15%-1.90%; the accuracy was 97.36%-103.23%, and the recoveries of the 6 compounds were 97.45%-103.93%. The content of each compound in A. sieboldii, as determined using the corresponding calibration curve, was in the range of 0.380-12.062 mg/g. This optimized simultaneous quantification method will be suitable for improving the quality control of A. sieboldii. Moreover, the 6 compounds in A. sieboldii showed an inhibitory effect on the production of chemokines, which suggests that A. sieboldii has an antiallergic effect.

由于当前用于治疗特应性皮炎（AD）（皮肤中的慢性疾病）的药物的副作用，传统草药作为替代疗法受到了广泛关注。传统上细辛细辛被用于治疗感冒，发烧，头痛，咳嗽，神经痛，慢性支气管炎，哮喘和过敏。在这项研究中，在6种化合物（松果菊苷，香草酸，kakuol，甲基丁香酚，芝麻素，和asarinin）A.天女进行同时分析使用高效液相色谱法（HPLC），和所提出的分析方法进行了验证。此外，西伯利亚曲霉的抑制作用在HaCaT细胞中评估提取物和6种分析化合物对趋化因子表达的影响。HPLC方法显示出高线性，相关系数≥0.9999。6种化合物的检出限为0.00至0.02 µg / mL，定量限为0.01至0.05 µg / mL。日内和日间精度为0.15％-1.90％；6种化合物的回收率分别为97.36％-103.23％和97.45％-103.93％。在每种化合物的含量A.天女如使用相应的校正曲线来确定，在的0.380-12.062毫克/克的范围内。这种优化的同步定量方法将适用于改善西伯利亚曲霉的质量控制。此外，西伯利亚A中的6种化合物尼古丁对趋化因子的产生具有抑制作用，这表明西伯利亚曲霉具有抗过敏作用。